We present a modeling and computational approach to study fusion of multicellular aggregates during tissue and organ fabrication, which forms the foundation for the scaffold-less biofabrication of tissues and organs known as bioprinting. It is known as the phase field method, where multicellular aggregates are modeled as mixtures of multiphase complex fluids whose phase mixing or separation is ...

Future biosensing applications will require high performance, including real-time monitoring of physiological events, incorporation of biosensors into feedback-based devices, detection of toxins, and advanced diagnostics. Such functionality will necessitate biosensors with increased sensitivity, specificity, and throughput, as well as the ability to simultaneously detect multiple analytes. Whil...

A biofabrication strategy for creating planar multiscale protein, hydrogel, and cellular patterns, and simultaneously generating microscale topographical features is developed that laterally confines the patterned cells and direct their growth in cell permissive hydrogels.

4D Biofabrication – a pioneering biofabrication technique involves the automated fabrication of 3D constructs that are dynamic and show shape-transformation capability. Although current methods highly promising for vascular elements such as tubes, tubular junctions is still challenging. Here, first time, biofabrication-based concept T-shaped bifurcation using printed shape-changing layers based...

Biofabrication commonly involves the use of liquid droplets to transport cells to the printed structure. However, the viability of the cells after impact is poorly controlled and understood, hampering applications including cell spraying, inkjet bioprinting, and laser-assisted cell transfer. Here, we present an analytical model describing the cell viability after impact as a function of the cel...

Signaling between cells guides biological phenotype. Communications between individual cells, clusters of cells and populations exist in complex networks that, in sum, guide behavior. There are few experimental approaches that enable high content interrogation of individual and multicellular behaviors at length and time scales commensurate with the signal molecules and cells themselves. Here we...

Biofabrication In article number 2204301, Marcy Zenobi-Wong and co-workers use Filamented Light (FLight) biofabrication to create hydrogel constructs containing ultrahigh-aspect-ratio microfilaments. FLight is both fast biocompatible. Microfilaments associated microchannels provide effective topological guidance cues instruct cell migration, alignment, extracellular matrix deposition of residen...

Many recent advances in bioprocessing have been enabled by developments in miniaturization and microfluidics. A continuing challenge, however, is integrating multiple unit operations that require distinct spatial boundaries, especially with included labile biological components. We have suggested "biofabrication" as a means for organizing cells and biomolecules in complex configurations while p...