Role of disulfide bridges in phytase's unfolding-refolding was probed using dynamic light scattering. Phytase was unfolded by guanidinium chloride and then refolded by removing the denaturant by dialysis. Thiol reagents prevented refolding; thus, disulfide bridge formation is an integral step in phytase folding. Catalytic demise of phytase after unfolding and refolding in presence of Tris(2-car...

A study was conducted to investigate the effects of thermostable phytase supplementation on the growth performance and nutrient digestibility of broilers for 42 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 6 pens of 20 chicks per pen. The control group was given maize-soybean meal basal diet and the other two groups were fed...

Phytase expressed and anchored on the cell surface of Pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. Furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst. In this study, the phytase gene of Citrobacter amalonaticus was fused with the Pichia pastoris glycosylphosphatidylinositol (GPI)-anchored glycoprotein h...

Two studies were conducted to determine the true P digestibility (TPD) of black-eyed pea (BEP) and peanut flour (PNF) and the TPD response to phytase supplementation using the regression method. Sequential diets containing 115, 230, or 345 g of BEP/kg (experiment 1) and 115, 230, or 345 g of PNF/kg (experiment 2) without or with 1,000 units of phytase/kg were formulated. Chromic oxide was added...

background: degradation of phytic acid to inorganic phosphate in domestic animals’ diets requires thermostable phytase. although basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. methods: the phytase gene from bacillus subtilis dr8886 was isolated...

Traditional supplemental dietary phytase loses activity during steam pelleting. The thermal tolerance and bioefficacy of a phytase product with a thermoprotective coating [coated phytase (C-phytase)] was compared in mash and pelleted diets to a traditional, uncoated phytase (U-phytase) added to a negative control (NC) diet, formulated with reduced dietary Ca and P, and compared with a corn-soyb...

Three experiments were conducted to test the hypotheses that pigs fed diets that are equal in digestible P will perform equally regardless of the concentration of total P in the diets, and that the addition of microbial phytase, distillers dried grains with solubles (DDGS), or a combination of phytase and DDGS will result in a reduction in P excretion. In Exp. 1, a P-free diet and 6 diets conta...

Two studies with growing pigs were conducted using the regression analysis technique to estimate the true digestibility of P (TPD) in canola (CM) and soybean (SBM) meals, and quantify the effect of microbial phytase on TPD of CM and SBM. In each study, 48 (Exp. 1) or 36 (Exp. 2) 17-kg barrows were assigned to 6 dietary treatments arranged in a 3 x 2 factorial of 3 graded levels of CM (Exp. 1) o...

Both 1,25-dihidroxyvitamin D3 and phytase are known to improve phosphorus utilization in broilers(Biehl and Baker,J Nutr 1997). Solanum glaucophyllum(Sg) is a plant containing high amounts of 1,25dihydroxyvitamin D bound to a glycoside. It may be an economical source of 1,25-dihydroxyvitamin D for animal feeds. We examined the effect of Sg, with and without phytase, on phosphorus utilization in...