An intercalator-linked macrocycle 3,6-bis(dimethylamino)-10-(6-[1,4,7-triazacyclononane]hexyl) acridinium bromide was synthesized, its copper(ii) complex was prepared, and the DNA cleavage activity of this metal complex was compared to that of the intercalator-free (Cu[9]aneN(3))(2+) complex.

With the goal of developing artificial nucleases for DNA hydrolysis, metal-coordinating peptides have been tethered to a DNA-intercalating rhodium complex to deliver metal ions to the sugar-phosphate backbone. The intercalator, [Rh(phi)(2)bpy']Cl(3) [phi = 9,10-phenanthrenequinone diimine; bpy' = 4-(butyric acid)-4'-methyl-2,2'-bipyridine], provides DNA binding affinity, and a metal-binding pep...

A bis-intercalator echinomycin (ECHI) and a simple intercalator [Co(phen)3]3+ were used as a novel electrochemical redox indicators to detect DNA hybridization at gold electrodes (AuE). In order to minimize the nonspecific adsorption of oligonucleotides (ODN), the thiol-derivatized oligonucleotides were immobilized onto AuE in the first step, and the exposition of AuE to 6-mercapto-1-hexanol (M...

Thermodynamic studies on the interactions between intercalator-neomycin conjugates and a DNA polynucleotide triplex [poly(dA).2poly(dT)] were conducted. To draw a complete picture of such interactions, naphthalene diimide-neomycin (3) and anthraquinone-neomycin (4) conjugates were synthesized and used together with two other analogues, previously synthesized pyrene-neomycin (1) and BQQ-neomycin...

DNA strand breaks produced by damaging agents such as x-ray generally stimulate poly(adenosine diphosphoribose) (ADP-R) synthesis in mammalian cells. DNA intercalating agents induce the formation of strand breaks which are unusual in that they are associated with tightly or covalently bound protein. In order to determine whether the intercalator-induced strand breaks are associated with poly-(A...

One of the important roles of a DNA sensor is the capability of detecting genetic diseases or mutations by analyzing DNA sequence. The electrochemical detection method can be simpler and cheaper than other methods available and hence this method was investigated in this paper. For a successful electrochemical detection, several aspects should be considered including chemical treatment of electr...

We have examined the dissociation of [N-MeCys3,N-MeCys7]TANDEM, an AT-selective bifunctional intercalator, from TpA sites in mixed-sequence DNAs by a modification of the footprinting technique. Dissociation of complexes between the ligand and radiolabelled DNA fragments was initiated by adding a vast excess of unlabelled calf thymus DNA. Portions of this mixture were subjected to DNAse I footpr...

A novel class of DNA cleavage agents are reported that derive activity from amino acids tethered to a photoactive intercalator.