Herpesvirus infections of domestic ruminants are about economic losses due to respiratory and reproductive problems. Bovine herpesvirus 1 (BHV-1) is a major pathogen of bovines world-wide and causes different clinical manifestations including infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis (IPV) / balanopostitis (IPB) and meningoencephalitis [3]. These conditions are...

A more closed farming system may prevent introduction of infectious diseases on dairy farms and can be a good starting point for eradication of these diseases. In an exploratory study it was calculated that being >open= had a negative influence on net profit of almost Dfl 1 per 100 kg of milk. A survey was carried out to make up an inventory of the existing more or less closed farming systems i...

This paper aims to evaluate different formats of the enzyme-linked immunosorbent assays (ELISAs) for detection of virus-specific antibodies and focuses on factors that may influence the diagnostic reliability of such tests. Newly developed and well-established ELISAs for detection of infections of bovine herpesvirus 1 (BHV1), bovine respiratory syncytial virus (BRSV), classical swine fever viru...

Various detection methods have been developed for serological monitoring BHV1, an alphaherpesvirus and the cause of the respiratory disease, abortion, conjectivitis and other clinical forms of disease complex in cattle, in different laboratories throughout the world. The objective of this study was to develop and evaluate dot-blot enzyme immunoassay as a proper and inexpensive method for detect...

Viral infections are ubiquitous in humans, animals, and plants. Real-time methods to identify viral infections are limited and do not exist for use in harsh or resource-constrained environments. Previous research identified that tissues produce unique volatile organic compounds (VOC) and demonstrated that VOC concentrations change during pathologic states, including infection, neoplasia, or met...

Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA) remains the most common analytical platform employed for serological analyses. The analysis of pooled samples instead of individual ones is a common procedure that permits to certify, with one single test, entire her...

Herpesvirus glycoprotein C (gC) is one of the major virus attachment proteins. Bovine herpesvirus type 1 (BHV-1) causes respiratory and genital diseases in cattle, whereas BHV-5 causes acute meningoencephalitis in calves. The gC gene sequence of these two viruses are substantially different. To determine the contribution of the BHV-5 glycoprotein gC (gC5) to the neuropathogenesis of BHV-5, we h...

Bovine respiratory disease (BRD) complex is a major disease, classically in the indoor calves and feedlot young cattle. The etiopathogenesis of BRD is multifactorial and complex. In this complex etiology an equivalent role plays both the infectious agents as well as the environmental factors which are called also as environmental stressors (inappropriate livestock management like mistakes in an...