Vacuoles were isolated from freshly cut slices of the storage roots of beetroot (Beta vulgaris), and from slices that had been washed in aerated water for 1-3 days. The unique vacuolar location of betanin permitted the use of a correlative method to determine whether sucrose and acid invertase were located in the vacuoles. The specific content (the activity of the enzyme or amount of substrate ...

Background: Polycystic ovary syndrome (PCOS) as a common cause of infertility is the endocrine disorder among reproductive-aged women. Nevertheless, the management and treatment of this major problem is not deterministic, and surrounded by many controversies. Objective: In this study the effects of betanin, as red pigment present in red beetroot, in the PCOS induced rats are evaluated. Method...

Mechanical wounding, infiltration with P. syringae or A. tumefaciens, and exposure to an H(2)O(2)-generating system (Glc/Glc oxidase) induce betacyanin synthesis in red beet (Beta vulgaris) leaves. These conditions also induced the expression of BvGT, a gene encoding a glucosyltransferase (GT) from Beta vulgaris. BvGT has a high similarity to Dorotheanthus bellidiformis betanidin-5 GT involved ...

Betalains are a family of natural pigments found exclusively in the plant order Caryophyllales. All members of this chemical family are biosynthesized through the common intermediate betalamic acid, which is capable of spontaneously condensing with various primary and secondary amines to produce betalains. Of particular interest is the red-violet betanin, most commonly obtained from Beta vulgar...

In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent orange 1∶1 complex with a stability constant of 1.4 × 10(5) L mol(-1). The addition of calcium dipicolinate, large...

The research on alternative natural colorants is of growing interest as substitutes for synthetic dyes in food. Opuntia lasiacantha Pfeiffer (red prickly pear) pigment was extracted from fruits of three different localities. The extracted pigment was identified as betanin by HPLC analysis comparing to the commercial beet red colorant E-162/1600. Color parameters and betanin content were also de...

Opuntia ficus-indica belongs to the Cactaceae family and is widespread in semi-arid and arid regions. Cactus pears are known for their health promoting properties which are due to a variety of bioactive molecules. This study aims to identify and quantify phenolic acids, flavonoids and betanin from spineless Opuntia ficus-indica fruits. Fresh mature samples were crushed, and extracted with 50% a...

The use of new synthesized ammonium-based ionic liquids was explored as an alternative to the current process implemented in betanin extraction from red beet juice, resulting high yields: 70% and 82%. Betanin is a vegetal pigment that has been applied large variety products food industry, which important, for it can work substitute synthetic dyes used nowadays. Additionally, kosmotropic salt so...