One of the main obstacles in providing quality potato seed tubers is long dormancy period seeds. A study that explores this has been carried out to elucidate response release and initiation sprouts differences tuber weight storage duration before immersion cytokinin (6-benzylaminopurine). The was conducted at warehouse UPT Horticulture Parent Seed Kutagadung Berastagi, Department Food North Sum...

In vitro organogenesis of citrus was studied for the genotypes Citrus sinensis cv. ‘Natal’, C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP – 0.0; 0.5; 1.0; 1.5, or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. auranti...

We previously showed that applying 10(-6) M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod(-)Myc(-)Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots. In contrast, inoculation with a Nod(-)S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (...

In vitro regeneration of plants from root cultures of Swertia chirata Buch.-Ham. ex Wall was obtained. Root explants from axenic shoot cultures were used for shoot induction. Optimal shoot regeneration without any callus intervention was observed on 1/2 MS (Murashige and Skoog’s medium, 1962) + 4.44 μM 6, benzylaminopurine (BAP) +1.07 μM α-naphthalene acetic acid (NAA).Regenerated shoots were f...

The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars – ‘Tulameen’ and ‘Black Jewel’ (Rubus idaeus L.), ‘Black Satin’ (Rubus fruticosus L.) and ‘Tayberry’ (Rubus fruticosus x Rubus idaeus). Nodal segments carrying dormant buds were used for in vitro culture establishment. For shoot initiation, MS medium containing 8 g*L phytoagar, 30 g*L sucrose, 1 mg*L ...

Sprouted corm sections of "South Dade" white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3-5 mm comprising the apical meristem with 4-6 leaf primordial, and approximately 0.5 mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg's B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA...

The accumulation o f betacyanin was reduced by the addition o f various cytokinins to sus­ pension cultures o f Phytolacca americana. The decrease in the accumulation o f betacyanin was overcome by exogenously supplied tyrosine which is a precursor o f betacyanin. Benzylaminopurine (BAP) decreased the level o f free tyrosine in the cells. Feeding experiments using labeled tyrosine revealed that...

An effi cient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortifi ed with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of hig...

A simple micropropagation method from shoot tip explants was reported here for Clitoria ternatea L., an economically important medicinal plant. High frequency of micro shoots were obtained from this explants on MS medium supplemented with various concentrations of Indole Acetic acid (IAA) (0.5 2.0 mg/l) and KN (0.5 -2.0 mg/l). Maximum number of shoots was obtained from shoot tip explants in the...

Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axil...