This article focuses on the importance of primary health care nurses' involvement in the identification of children with severe disabilities, early and appropriate intervention that includes referral, as well as the provision of support to the children's caregivers. The use of multiskilling as a strategy to train nurses to fulfil this role is described. The traditional roles of community nurses...

In this work, automatic pH changing system on lab-on-paper devices for enzyme-linked immunosorbent (ELISA) has been developed in order to improve their sensitivity and make the technique much more applicable to real samples. The most widely used enzyme in immunoassay are alkaline phosphatase (ALP) and peroxidase. ALP-conjugated secondary detection with BCIP/NBT substrate provides high sensitivi...

Two-color fluorescent in situ hybridization (FISH) is a widely used technique for comparing relative gene expression patterns. Current two-color FISH protocols are not ideal for detecting weakly expressed transcripts or monitoring signal strength and background levels during the course of the reaction. Here we describe an improved FISH protocol using the conventional highly sensitive chromogeni...

Two-color whole-mount in situ hybridization is a powerful technique for comparing the spatial expression patterns of two genes in developing embryos or within a given adult tissue. Many current procedures use two genespecific antisense RNA probes, each incorporating a different chemical moiety that is recognized by a specific antibody. Typically, the first gene-specific RNA probe is labeled wit...

This corrects the article DOI: 10.1038/srep45235.