By combining the principles of density gradient separation and two phase partitioning, we devised a system to obtain highly pure plasmalemma and tonoplast vesicles from citrus (Citrus limettioides Tan.) juice cells. Both tonoplast and plasmalemma fractions were virtually free from golgi, endoplasmic reticulum and mitochondria contamination. Plasmalemma and tonoplast samples were also clean from...