An enzyme-linked immunosorbent assay was used to detect and quantitate the parasporal crystal toxins of Bacillus thuringiensis subspp. kurstaki and israelensis. The assay method described is extremely sensitive, accurate, and highly specific. With this technique, crystalline insecticidal proteins from several subspecies of B. thuringiensis were compared. The dipteran crystal toxin produced by B...

Levels, genetic traits and stability of resistance to Bacillus thuringiensis of the Bang Bua Tong (BS) population of the diamondback moth Plutella xylostella collected in Thailand were investigated. The BS population exhibited 668 and 66.5-fold resistance to formulations derived from B. thuringiensis subsp. kurstaki (Toarow CT®), and B. thuringiensis subsp. aizawai (XenTari®), respectively. In ...

This study was conducted to determine the suitable time to apply the Bacillus thuringiensis sub species kurstaki against the larvae of the Metisa plana oil palm. The aim of this study is to search for the most economical method for using the B. thuringiensis sub species kurstaki. Owing to that matter, the larvae was sprayed at three different times, 0800, 1200, and1600, with 0.68ml of the B. th...

Insecticides are important in agriculture, to reduce human disease, and decrease the nuisance of biting insects. Despite this, many have potential for environmental impacts toxicity nontarget organisms. We reviewed data on effects insecticides based toxins from Bacillus thuringiensis var. israelensis (Bti) kurstaki (Btk) amphibians. The few peer-reviewed publications that available Bti provide ...

A population (SERD3) of the diamondback moth (Plutella xylostella L.) with field-evolved resistance to Bacillus thuringiensis subsp. kurstaki HD-1 (Dipel) and B. thuringiensis subsp. aizawai (Florbac) was collected. Laboratory-based selection of two subpopulations of SERD3 with B. thuringiensis subsp. kurstaki (Btk-Sel) or B. thuringiensis subsp. aizawai (Bta-Sel) increased resistance to the se...

We report the draft genome sequences of two insecticidal strains against lepidopteran pests, Bacillus thuringiensis serovar kurstaki strain BP865, an isolate from the South Korean phylloplane, and strain HD-133, a reference strain of B. thuringiensis serovar aizawai.

Sau 3A1 partial digestion fragments from Bacillus thuringiensis var. kurstaki HD-1 plasmid DNA were ligated into the BamHI site of the cloning vector pBR322 and transformed into Escherichia coli strain HB101. Colonies presumed to contain recombinant plasmids were screened for production of an antigen that would react with antibody made against B. thuringiensis crystals. One strain, ES12, was is...

We report here the complete genome sequence of Bacillus thuringiensis subsp. kurstaki strain HD-1, which serves as the primary U.S. reference standard for all commercial insecticidal formulations of B. thuringiensis manufactured around the world.