lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. in this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of isfahan province (iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. to select a the...

The amino acid sequence of the RNA polymerase (RNAP) α-subunit is well conserved throughout the Eubacteria. Its C-terminal domain (α-CTD) is important for the transcriptional regulation of specific promoters in both Escherichia coli and Bacillus subtilis, through interactions with transcription factors and/or a DNA element called the "UP element". However, there is only limited information rega...

Th e eff ects of various carbon and nitrogen sources on production of α-amylase by Bacillus subtilis and its mutant derivates were investigated. Th e maximum production of α-amylase by all strains was obtained in the presence of mesoinositol as the carbon source. Th ere was no more signifi cant increase in enzyme yield in the case of the supplementation of nitrogen sources, whereas malt extract...

conclusions the present study suggests that the enzyme obtained from strain b. subtilis can be used as feed supplement in animal diet also for reduction of phosphorus pollution problem in areas of livestock production. materials and methods the present study was conducted in biotechnology laboratory, motilal nehru national institute of technology, allahabad, uttar pradesh, india. to isolate phy...

background: bacillus subtilis refers to stretched and sometimes curved, gram-positive, aerobic, and catalase-positive bacilli, which has thermo-resistant endospores. it has been known as a normal flora in the human but can be pathogens in the case of opportunistic. also, it can be the pathogen of nosocomial infections such as wound among hospitalized patients. purpose of this study was to ident...

Staphylococcus aureus is one of the prominent Gram positive human pathogen secretes many surface and secretary proteins including various enzymes and pathogenic factors that favour the successful colonization and infection of host tissue. α-amylase is one of the enzymes secreted by S. aureus which catalyses the breakdown of complex sugars to monosaccharides, which are required for colonization ...

The hyperthermophilic α-amylase from Thermococcus sp. HJ21 possesses unique traits (Ca(2+)-independent thermostability and optimal temperature of 95°C) that make it a great potential candidate for use in the food industry. However, this Archaea isolated from a deep-sea thermal vent requires strict control of culture conditions and produces only small amounts of α-amylase. To solve these problem...

α-amylases are one of the most common and important industrial enzymes widely used in various industries. The present study was conducted with aim isolating identifying symbiotic α-amylase enzyme-producing bacteria intestine Silago Sihama Rasterliger Canagorta fish living Qeshm Island, Hormozgan. intestinal these species were isolated using nutrient agar culture medium; then, producing screened...

Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In this question-oriented review, every effort is made to answer the following que...