The limitations of fungal laccases at higher pH and salt concentrations have intensified the search for new extremophilic bacterial laccases. We report the cloning, expression, and characterization of the bacterial cotA from Bacillus clausii, a supposed alkalophilic ortholog of cotA from B. subtilis. Both laccases were expressed in E. coli strain BL21(DE3) and characterized fully in parallel fo...

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

Bacillus clausii TnrA transcription factor is required for global nitrogen regulation. In order to obtain anoverview of gene regulation by TnrA in B. clausii KSMK16, the entire genome of B. clausii was screened forthe consensus sequence, 5’-TGTNAN7TNACA-3’ known as the TnrA box, and 13 transcription units werefound containing a putative TnrA box. The TnrA targets identified in...

Here, the olive leaf extract (OLE) rich in polyphenols was employed as a prebiotic agent, together with Lactobacillus reuteri and Bacillus clausii, to develop synbiotics. The effect of on probiotic strains tested at concentrations 0, 50, 100, 400, 1000 μg mL−1, also 20 40 mg mL−1. Olive mL−1 showed best activity L. B. clausii. A basal diet two experimental synbiotic-containing diets were prepar...

Probiotic milk has been previously demonstrated to reduce the number of respiratory infections (RI) among children attending day care centres. Thus, this pilot study was aimed to assess the efficacy and the safety of 3 month treatment with Bacillus clausii in the prevention of recurrent respiratory infections (RRI) in children. Eighty children with RRI were studied: 40 of them were randomly tre...

In this 30-day experiment, the effect of probiotics (Bacillus clausii, commercial probiotics: Enterogermina) and prebiotics (Galactooligosaccharides, GOS) on growth red cherry shrimp (Neocaridina davidi) has been investigated for 30 days. The trial was conducted with 270 shrimps (initial weight: 0.24±0.03 g) in 27 plastic tanks (volume 0.01 m3 area 0.05 m2) representing 9 groups. Except control...

The clinical benefits observed with probiotic use are mainly attributed to the antimicrobial substances produced by probiotic strains and to their immunomodulatory effects. Currently, the best-documented probiotic bacteria used in human therapy are lactic acid bacteria. In contrast, studies aiming to characterize the mechanisms responsible for the probiotic beneficial effects of Bacillus are ra...

Oral probiotics containing spores of Bacillus clausii are given to the patients who have undergone major surgery or on long term antibiotic course, restore intestinal microbial flora. We reporting a rare case where oral probiotic suspension was injected intravenously by mistake. shall be discussing in detail reactionary changes caused erroneous injection body, its effects, complications and tre...