Background: IseA from Bacillus subtilis is an inhibitor protein against DL-endopeptidases including synthetic lethal autolysins. Results: We solved the solution structure of IseA using NMR. Titration with LytF DL-endopeptidase indicated interaction sites around the loop region. Conclusion: The IseA structure revealed a novel “hacksaw”-like fold with a characteristic inhibitory loop. Significanc...

Low concentrations of glutaraldehyde (0.1% or higher) blocked cellular and wall autolysis. The site of autolytic activity was studied by allowing cell autolysis to proceed for very short periods (0 to 15 min) before addition of glutaraldehyde. Electron microscopy of ultrathin sections showed that the primary site of autolytic activity was the leading edge of the nascent cross wall. The base of ...

pneumococcal autolysins are enzymes involved in cell wall turnover and cellular division physiologically. they have been found to be involved in the pneumococcus pathogenesis. the aim of this study was to identify the autolytic activity of spr1754 as a novel protein of streptococcus pneumoniae . moreover, the binding of the recombinant protein to plasma proteins was also determined. the spr1754...

background and objectives: streptococcus agalactiae is the leading cause of bacterial sepsis and meningitis in newborns and results in pneumonia and bacteremia in adults. a number of s. agalactiae components are involved in colonization of target cells. destruction of peptidoglycan and division of covalently linked daughter cells is mediated by autolysins. in this study, autolytic activity and ...

The lysis of Staphylococcus aureus FDA 209P induced by benzylpenicillin was completely inhibited by cellular lipoteichoic acid isolated from an homologous organism. The cells prevented from penicillin-induced lysis were in static state and did not lose viability. The lipoteichoic acid inhibited either extracellular autolysin activity in culture supernatant or autolysis of whole cells in exponen...

BACKGROUND The role played by pneumolysin and autolysin in pneumococcal meningitis is poorly understood. METHODS A rat model was used to investigate the disease, in which surgical implantation of a cisternal catheter allowed bacterial instillation and cerebrospinal fluid (CSF) sampling. RESULTS CSF infection of rats with wild-type pneumococci caused meningitis within 26 h, whereas isogenic ...

Mutants of Streptococcus pneumoniae that contain active autolysin and yet cannot be induced to lyse during treatment with penicillin (Lyt+Tol+ mutants) have been described. We have now shown that these mutants are temperature dependent (32 "C); at 37 "C these bacteria underwent penicillin-induced lysis. In addition, mutants at the lysis-permissive temperature showed the socalled 'paradoxical re...

A Kuroda and J Sekiguchi by a sin (flaD) mutation. expression of the sigma D gene and is affected subtilis autolysin operon depends on High-level transcription of the major Bacillus

Cell wall turnover was examined in parent and mutant strains of Staphylococcus aureus. Peptidoglycan and teichoic acid were observed to undergo turnover in the wild-type strain during exponential growth; however, the rate of turnover did not decrease when the growth rate slowed, as the culture entered stationary phase. Isolated native cell walls and crude soluble autolytic enzyme were prepared ...