A study was initiated to construct a micro-reactor for protein digestion based on trypsin-coated fused-silica capillaries. Initially, surface plasmon resonance was used both for optimization of the surface chemistry applied in the preparation and for monitoring the amount of enzyme that was immobilized. The highest amount of trypsin was immobilized on dextran-coated SPR surfaces which allowed t...

Proteomics is the large-scale study of proteins, particularly their stuructures and functions. One of the most important point is to develop efficient and rapid approachehes to identify the target proteins. Peptide mass mapping and tandem mass spectrometry (MS/MS)-based peptide sequencing are key methods in current protein identification for proteomic studies. Proteins are usually digested into...

Inflammation of pancreatic parenchyma is said to be pancreatitis. It can be acute, presenting as emergency with short history or chronic as a continuation of acute with a long history. Autodigestion is responsible for pancreatitis on background of premature activation of pancreatic enzymes within the pancreas. Acute Pancreatitis is acute inflammation of the pancrease which can be mild to modera...

To automatically annotate songs with descriptive keywords, a variety of content-based auto-tagging strategies have been proposed in recent years. Different approaches may capture different aspects of a song’s musical content, such as timbre, temporal dynamics, rhythmic qualities, etc. As a result, some auto-taggers may be better suited to model the acoustic characteristics commonly associated w...

A 10 years old insulin dependent diabetic male child presented with gradual blackening of penis and ketoacidosis after incision and drainage of abscess at penile shaft under penile ring block with xylocaine with adrenaline. Patient was managed for diabetes, debridement and SPC was done but process of gangrene continued; finally whole penis slough out. Perineal urethrostomy was done and patient ...

anaerobic digestion is a feasible technology to stabilize the solid waste generated inslaughterhouses obtaining significant quantities of biogas, considered as a clean and renewable fuel. this iswhy it is important to optimize the digestion process in order to eliminate organic matter and maximize theproduction of biogas. in this work, a system was developed for treating slaughterhouse solid wa...

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewat...