Antisera to nucleoli of Novikoff hepatoma ascites and normal rat liver cells were produced in rabbits by injection of whole, isolated nucleoli. These antisera have been used to compare the nucleolar antigens that were partially fractionated by differential solubilization from nucleoli. Fourteen antigens were detected by these antisera; ten of these antigens were detected by both antisera. Oucht...

SUMMARY Antinucleolar antisera were produced in rabbits immunized with whole isolated nucleoli from normal rat liver and rat Novikoff hepatoma ascites cells. These antisera produced positive nucleolar fluorescence of varying degrees in nucleoli and nuclei of Novikoff tumor, rat liver, kidney, and Walker tumor cells. Novikoff hepatoma and liver antinucleolar antisera fixed complement when combin...

The effects of niacin, namely, nicotinic acid and nicotinamide, and trigonelline on the proliferation and invasion of cancer cells were studied using a rat ascites hepatoma cell line of AH109A in culture. Niacin and trigonelline inhibited the invasion of hepatoma cells at concentrations of 2.5-40 microM without affecting proliferation. Hepatoma cells previously cultured with a reactive oxygen s...

Ascitic bile acids in a cirrhotic patient with hepatoma were determined by gas liquid chromatography. The bile acids in ascites were considered to originate from those in hepatic lymph transuded by postsinusoidal obstruction in liver cirrhosis.

Antinucleolar antisera were produced in rabbits immunized with whole isolated nucleoli from normal rat liver and rat Novikoff hepatoma ascites cells. These antisera produced positive nucleolar fluorescence of varying degrees in nucleoli and nuclei of Novikoff tumor, rat liver, kidney, and Walker tumor cells. Novikoff hepatoma and liver antinucleolar antisera fixed complement when combined with ...

Nucleoli were isolated by sonication and differential centrifugation from Novikoff hepatoma ascites cells, Ehrlich ascites cells, HeLa cells, and H-50 cells. RNA was extracted with the sodium doclecyl sulfate-phénolprocedure and fractionated by sucrose density (¡radientcentrifugation, which revealed that the nucleolar RNA could be fractionated into 4 major components with sedimentation consta...

avoiding cytolysis or membrane disruption. After incuba tion, the mixtures were centrifuged, and 105,000 x g supernatants were subjected to gel filtration. The macromolecular peak fractions thus obtained contained about 2% radio activity of the whole cells or 50% radioactivity of the plasma membranes. They showed several physical and chemical features characteristic of acid mucopolysaccharides....

The insulin receptors in two strains of Zajdela ascites hepatoma cells have been studied. Specific binding of insulin was investigated at 4,15, and 22°,and the amount of insulin bound at steady state was proportional to the cell concentration. The optimum pH was 7.6. Scatchard analysis of the insulin binding exhibited cur vilinear plots. The number of insulin receptors was mark edly reduced in...

Several t ransplantable tumors and chick embryonic tissues were cultured in collagen-coated cellulose sponge. The growth obtained in this matr ix was compared with that obtained in cellulose sponge alone and in collagen sponge alone. The tumors included a mouse mammary adenoeareinoma (C3HBA), a squaincus cell carcinoma of the mouse cervix (D-98), a mouse ascites hepatoma (Sato A H #134) , a rat...

The antitumor activity of 2,3-dihydroxybutyraldehyde on Ehrlich carcinoma, Sarcoma 180, and Yoshida AH 130 hepatoma, as well as the aldehyde dehydrogenase activity in these tumors, was studied. 2,3-Dihydroxybutyraldehyde at nontoxic doses (500 mg/kg body weight i.p. daily for 7 days) slowed down the growth of solid and ascites tumors in mice. The treatment completely prevented the development o...