As public health agencies struggle to track and contain emerging arbovirus threats, timely and efficient surveillance is more critical than ever. Using historical dengue data from Puerto Rico, we developed methods for streamlining and designing novel arbovirus surveillance systems with or without historical disease data.

Hyalomma marginatum is a hard-bodied tick found on animals. This tick has been implicated in the transmission of Bahig virus, a pathogenic arbovirus previously thought to be transmitted only by mosquitoes.

The emergence of the West Nile virus (WNV) in the northeastern United States has drawn emphasis to the need for expanded arbovirus surveillance in Connecticut. Although the state of Connecticut began a comprehensive mosquito-screening program in 1997, only since 1999 have there been efforts to determine the prevalence of arboviruses in bird populations in this state. Herein, we report on our re...

Plaque development of 42 arbovirus serotypes, representing 17 serogroups, was studied in a clonal line (PS Y-15) of porcine kidney. The arboviruses plaqued within 3 to 7 days, with plaque diameters ranging from 1 to 5 mm. Mouse mortality and cell infectivity end points were compared with 24 arboviruses. Arbovirus LD(50) end points in mice exceeded the porcine kidney monolayer plaque-forming uni...

RNA interference (RNAi) was shown over 18 years ago to be a mechanism by which arbovirus replication and transmission could be controlled in arthropod vectors. During the intervening period, research on RNAi has defined many of the components and mechanisms of this antiviral pathway in arthropods, yet a number of unexplored questions remain. RNAi refers to RNA-mediated regulation of gene expres...

In most instances, vaccination is accepted to be the most effective method of preventing Culicoides-borne arbovirus transmission, as it has proven to be successful in large-scale campaigns. Under certain scenarios, however, vaccines require time to be developed and deployed or are not used due to financial, logistical or trade constraints. In the absence of vaccines, animal movement restriction...

Arboviruses are transmitted by distantly related arthropod vectors such as mosquitoes (class Insecta) and ticks (class Arachnida). RNA interference (RNAi) is the major antiviral mechanism in arthropods against arboviruses. Unlike in mosquitoes, tick antiviral RNAi is not understood, although this information is important to compare arbovirus/host interactions in different classes of arbovirus v...