microbe-associated molecular patterns (mamps) play key roles as activators of the innate immune response in animals and, analogously, as elicitors of defense responses in plants. however, there are not much investigations concerning their real importance in plant resistance against pathogens. here we used derivatives of flagellin and ef-tu as two well-known mamps against some important fungal a...

Trehalose (a-D-glucosyl-[1,1]-a-D-glucopyranoside) is involved in mechanisms that coordinate metabolism with plant growth adaptation and development. The main objective of the current work was to find out whether trehalose feeding affects the expression of two genes involved in photosynthesis: one gene coding for photosystem1 subunit H (PS1-H) and the other for the light harvesting complex B1 (...

to explore the function of light-harvesting complex protein (lhcp) in arabidopsis growth and development, the leclere and bartel seed collection was screened. in this collection randomly cloned cdnas are expressed under the camv35s promoter. a pale green line has been identified and characterized in more details. analysis of the inserted cdna in the pale green line showed it encodes lhcb1 prote...

there is no functional annotation for the majority of the several hundreds of receptor-like kinases in plants. a direct way of inferring the function of these proteins is to study the phenotype that results from loss of function mutants such as t-dna mutant lines. in this research a function (phenotype) to at2g37050 gene that encodes a receptor like kinase in arabidopsis t-dna line was assigned...

the hydrophobic proteins of plant plasma membrane still remain largely unknown.  for example in the arabidopsis genome, receptor-like kinases (rlks) are plasma membrane proteins, functioning as the primary receptors in the signaling of stress conditions, hormones and the presence of pathogens form a diverse family of over 610 genes. a limited number of these proteins have appeard in protein pro...

seth4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of arabidopsis thaliana. this fragment was pcr amplified using kod hi fi dna polymerase enzyme. this fragment was cloned into pgbkt7 bate vector and transformed e. coli dh5? cells containing vector were selected on lb medium containing kanamycin. finally, pgbkt7-seth4 bate was transformed into yeast ...

SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...

The Arabidopsis thaliana CORONATINE INSENSITIVE1 (COI1) gene encodes an F-box protein to assemble SCF(COI1) complexes essential for response to jasmonates (JAs), which are a family of plant signaling molecules required for many essential functions, including plant defense and reproduction. To better understand the molecular basis of JA action, we screened for suppressors of coi1 and isolated a ...

The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family members are plant unique serine/threonine kinases which play a key role in cellular signaling in response to abiotic stresses. The three SnRK2 members including SRK2D, SRK2I and SRK2E are known to phosphorylate major abscisic acid (ABA) responsive transcription factors, ABF2 and ABF4, involved in an ABA-dependent stress signal...