Many genes are hypothesized to be involved in determining an individual's risk for coronary artery disease (CAD). Recent efforts have focused on the genetic architecture of quantitative traits related to risk for CAD. Studies relating genetic variation in the structural gene for apolipoprotein E to plasma levels of apolipoprotein E illustrate strategies to begin to understand the genetic archit...

background: we aimed here to investigate the association between alleles and genotypes of apoe and age-related macular degeneration (amd) development. materials and methods: after ophthalmological examination, 120 patients with confirmed amd and 120 healthy controls were enrolled in the study. the polymorphic segment of apoe gene was pcr-amplified and sequenced to determine the frequency distri...

Radioiodinated apolipoprotein E, added in small amounts to rat serum, rapidly associates with the serum very low density lipoproteins and high density lipoproteins in proportion to their content of endogenous and apolipoprotein E. The labeled lipoproteins can be separated, without ultracentrifugation, by molecular sieve chromatography. When these labeled lipoproteins are injected intravenously ...

A radioimmunoassay for apolipoprotein E in human blood serum has been developed that measures equally the major polymorphic species of the protein (apolipoproteins E-1, E-2, E-3, and E-4) and the apo E in the dimer of apolipoproteins E and A-II. The assay is specific and yields values for apolipoprotein E in very low density lipoproteins that agree closely with those obtained by a quantitative ...

Apolipoproteins B and E were determined in 40 patients with type III hyperlipoproteinaemia (familial dysbetalipoproteinaemia) and in 48 patients with other types of hyperlipoproteinaemia matched for cholesterol and triacylglycerols. In type III patients, apolipoprotein E was increased and apolipoprotein B was lower than in other types of hyperlipoproteinaemia. The apolipoprotein E to apolipopro...

The expression of apolipoprotein E in cultured neonatal rabbit aortic smooth muscle cells was examined. Northern blot analysis determined that there was a single RNA transcript of approximately 1.2 kb. Moreover, a polyclonal antibody against rabbit apolipoprotein E was prepared in a goat and used in immunoprecipitations to demonstrate that the cultured cells secreted apolipoprotein E into the m...

High density lipoproteins of rat blood plasma were labeled in vitro with radioiodinated apolipoprotein E and biologically with [3H]cholesteryl esters. These two components, present in high density lipoproteins separated from serum of normal or cholesterol-fed rats by molecular sieve chromatography, were removed slowly from perfused livers and the labeled apolipoprotein E was also removed slowly...

multiple sclerosis (ms) is a chronic inflammatory disorder of the central nervous system, with a complex etiology that includes a strong genetic component. the chromosome 19q 13 region surrounding the apolipoprotein e (apoe) gene has shown consistent evidence of involvement in ms. in a cross-sectional study, to show the apoe genotype and allele frequency in the ms population of iran in comparis...

background: nonalcoholic fatty liver disease (nafld) is the most common chronic liver disease in the world. oral administration of symbiotic and vitamin e has been proposed as an effective treatment in nafld patients. this study was carried out to assess the effects of symbiotic and/or vitamin e supplementation on liver enzymes, leptin, lipid profile, and some parameters of insulin resistance (...

Lipoprotein glomerulopathy is a rare kidney disease in which lipoprotein thrombi are seen in the glomerular capillaries. Most of these patients are found in Japan and East Asian countries. The presenting symptoms include proteinuria, an abnormal plasma lipoprotein profile that resembles type III hyperlipoproteinaemia, and a marked increase in serum apolipoprotein E concentration. Previous studi...