The stabilization of a test plasmid by the proteic, poison-antidote plasmid addiction system (pas) of plasmid pTF-FC2 was host strain dependent, with a 100-fold increase in stability in Escherichia coli CSH50, a 2.5-fold increase in E. coli JM105, and no detectable stabilization in E. coli strains JM107 and JM109. The lethality of the PasB toxin was far higher in the E. coli strains in which th...

parD and chpA are homologous conditional killer systems of plasmid and chromosomal origin, respectively, encoding a killer protein (Kid and ChpAK) and an antidote (Kis and ChpAI). Here it is shown that these systems can functionally interact. A multicopy chpA recombinant partially complements two mutations in the antidote of the parD system. These mutations affect either autoregulation or neutr...

resume of the results arrived at by Aron, with chlorine as an antidote, will be necessary. Aron, in the paper already quoted, refers to the employment as antidotes, of alcohol, atropine, caffeine, ozone, potassium permanganate and chlorine. In connection with the use of chlorine Aron remarks ? "By the exceedingly satis" factory results arrived at by Lenz from the " use of chlorine as an antidot...

BACKGROUND Acutely poisoned patients sometimes require immediate treatment with an antidote, and delays in treatment can be fatal. We sought to determine the availability of 10 antidotes at acute care hospitals in Ontario. METHODS Mailed questionnaire with repeated reminders to pharmacy directors at all acute care hospitals in Ontario. RESULTS Responses were obtained from 179 (97%) of 184 h...

The P1 plasmid addiction operon encodes Doc, a toxin that kills plasmid-free segregants, and Phd, an unstable antidote that neutralizes the toxin. Additionally, these products repress transcription of the operon. The antidote binds to two adjacent sites in the promoter. Here we present evidence concerning the regulatory role of the toxin, which we studied with the aid of a mutation, docH66Y. Th...

Previews by catalyzing the removal of iodine from the inner ring. When the function of D3 was inactivated by iopanoic acid (IOPA), a specific inhibitor of the enzyme, the asym-metric growth pattern in response to thyroid hormone What if you woke up one morning and your eyes were was lost, and both dorsal and ventral CMZ grew equally. on the top of your head? You would be confused to see In comp...

antidote was repeated several times, ?without any apparent effect, and the rabbit died before the other which had no antidote. The second rabbit, -when oil the point of death, quite unconscious, and with feeble convulsive movements commencing, had its trachea opened and artificial respiration applied. There was no return of consciousness or of voluntury movement ; the tail was observed to move,...