background: human exposure to antibiotic resistant bacteria (arb) is a public health concern which could occur in a number of ways. wastewaters seem to play an important role in the dissemination of bacteria and antibiotic resistant genes (args) in our environment. the aim of this study was to evaluate the occurrence of three groups of arb and their resistance genes in hospital and municipal wa...

Wastewater discharged from clinical isolation and general wards at two hospitals in Singapore was examined to determine the emerging trends of antibiotic resistance (AR). We quantified the concentrations of 12 antibiotic compounds by analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS), antibiotic-resistant bacteria (ARB), the class 1 integrase gene (intI1), and 16 antibioti...

Plasmids operate as independent genetic elements in microorganism communities. Through horizontal gene transfer (HGT), they can provide their host microorganisms with important functions such as antibiotic resistance and heavy metal resistance. In this study, six metagenomic libraries were constructed with plasmid DNA extracted from influent, activated sludge (AS) and digested sludge (DS) of tw...

conclusions our findings revealed that the zfn technology could be employed to overcome ampicillin resistance by the targeted disruption of the ampicillin resistance gene, which leads to inactivation of β-lactam synthesis. therefore, zfn technology could be engaged to decrease the antibiotic resistance issue with the construction of a zfn archive against different args. to tackle the resistance...

Wastewater treatment plants are one of the main sources of dissemination of antibiotic resistance genes (ARGs) into the environment. The present study was conducted to determine the prevalence and removal of ARGs in different wastewater treatment processes. A total of 36 samples from raw and final effluent of different activated sludge processes were collected and analyzed. Molecular analysis w...

Copper is one of the most abundant heavy metals present in swine manure. In this study, a laboratory-scale aerobic composting system was amended with Cu at three levels (0, 200, and 2000 mg kg-1, i.e., control, Cu200, and Cu2000 treatments, respectively) to determine its effect on the fate of copper resistance genes [copper resistance genes (CRGs): pcoA, cusA, copA, and tcrB], antibiotic resist...

T he dissemination of antibiotic resistance genes (ARGs) carried by antibiotic-resistant bacteria (ARB) and their threats to public health have attracted concerns worldwide in the recent years (1). A number of organizations and governments have enhanced their monitoring schemes of ARB and ARGs, including the World Health Organization (2), the Public Health Agency of Canada, the Houses of Parlia...

The aim of this study was to investigate selective pressure of antibiotics on antibiotic resistance genes (ARGs) and bacterial communities in manure-polluted aquatic environment. Three treatment groups were set up in freshwater-sediment microcosms: tetracyclines group, sulfonamides group and fluoroquinolones group. Sediment and water samples were collected on day 14 after treatment. Antibiotic ...

BACKGROUND Soil bacteria naturally produce antibiotics as a competitive mechanism, with a concomitant evolution, and exchange by horizontal gene transfer, of a range of antibiotic resistance mechanisms. Surveys of bacterial resistance elements in edaphic systems have originated primarily from human-impacted environments, with relatively little information from remote and pristine environments, ...