In contrast to neutrophils or B-lymphocytes, cells of the monocytic lineage like rat macrophages, human peripheral blood monocytes and Mono Mac 6 cells contain a strong inhibitor of 5-lipoxygenase (5-LO) activity, which scavenges hydroperoxides and inhibits 5-LO activity in broken-cell preparations in the absence of exogenously added thiols. Chromatographic purification of the inhibitor from th...

Introduction: The fate of xenobiotics that is present, increasing day by day. The increasing fates altered or inhibit the metabolic activities like detoxification and biotransformation. Methods: The present study highlights this slow biotransformation and detoxification on the basis of specific enzymes which have a say in assessment of mercuric chloride toxicity and modulation by Panax ginse...

Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed by high light intensity, high salinity, or application of methylviologen or t-...

Glutathione peroxidase (GPX) is a critical antioxidant selenoenzyme in organisms that protects cells against oxidative damage by catalyzing the reduction of hydroperoxides by glutathione (GSH). Thus, some GPX mimics have been generated because of their potential therapeutic value. The generation of a semisynthetic selenoenzyme with peroxidase activity, which matches the catalytic efficiencies o...

Benzo[d]isoselenazol-3-ones N-substituted with sterically hindered diamagnetic and paramagnetic five- or six-membered nitroxides or their precursors, including ring-opened diselenides, exhibit synergism in glutathione peroxidase (GPx) activity.

The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phytoplanktivorous silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ctenopharyngodon idellus). Using genome walker method, a 750 bp 5'-flanking region of the silver carp...

In this study, some biochemical responses of Cyfluthrin (CFT), a commercial insecticide in Dreissena polymorpha, were investigated. The 96 hour LC50 value CFT on D. polymorpha was calculated as 553.22 ± 27.3 µg / L. exposed to subletal concentrations (1/20, 1/10 and 1/5 value) for 24 hours. enzyme activities superoxide dismustase (SOD), catalase (CAT) glutathione peroxidase (GPx) determined ind...

introduction : recurrent aphthous stomatitis (ras) is a common disease with unknown etiology and no treatment. the purpose of this study is to assess the relationship between serum antioxidants level and clinical manifestation. change in antioxidant level can lead inflammatory reaction through oxidative stress. materials & methods : a total 50 patients were selected for this cross sectional stu...

Both radiation and anthracycline antibiotics may produce reactive oxygen species to cause cytotoxicity, and it has been suggested that some cellular antioxidant enzymes may be important for resistance to these agents. The human breast adenocarcinoma cell line MCF-7WT has a low level of glutathione peroxidase (GPX) activity. We have transfected MCF-7WT cells with a plasmid that contains the cDNA...