This study contributes to the improvement of low-cost biotechnology for wastewater treatment in constructed wetlands (CWs). Constructed wetlands are energy efficient engineered systems that mimic the treatment processes of natural wetlands, removing polluting organic matter, nutrients, and pathogens from water. The aim of this study was to investigate the advisability of the inoculation of hori...

Heat-injured cells of Listeria monocytogenes were recovered from heated raw milk containing noninjured Enterococcus faecium by combining a simple method for obtaining strict anaerobiosis with a novel enrichment broth, Penn State University broth (PSU broth). Strictly anaerobic conditions were rapidly achieved by adding 0.5 g of filter-sterilized cysteine per liter to PSU broth and then purging ...

Methyl tert-butyl ether (MTBE), an octane enhancer and a fuel oxygenate in reformulated gasoline, has received increasing public attention after it was detected as a major contaminant of water resources. Although several techniques have been developed to remediate MTBE-contaminated sites, the fate of MTBE is mainly dependent upon natural degradation processes. Compound-specific stable isotope a...

A plethora of organic micropollutant mixtures are found in untreated municipal wastewater. Anaerobic digesters receive large loadings of hydrophobic micropollutants that sorb to wastewater biosolids. Despite micropollutants being pervasive as mixtures, little research is available to explain the impact that mixtures of compounds, as well as exposure time, have on microbial communities in anaero...

Background and Objective: Clostridium perfringens is an anaerobic bacterium, commonly present in retail foods. Its enterotoxin-producing ability, short generation time, ability to grow at elevated temperatures, and spore-forming ability, allows it to survive in food-processing temperatures, and cause foodborne illness. The aim of study was to screen dehydrated vegetables contaminated with cpe a...

Although many polycyclic aromatic hydrocarbons (PAHs) are known to be biodegraded under aerobic conditions, most contaminated sediments are anaerobic. With recent results demonstrating that some bicyclics and PAHs can be degraded without oxygen, information on specific biodegradation rates and electron acceptor stoichiometry is lacking. A fluidized bed reactor (FBR) enrichment approach was used...

Crude oil is a complex mixture mainly composed of various saturated and aromatic hydrocarbons. Whereas degradation of hydrocarbons by oxygen-respiring microorganisms has been known for nearly one century, utilization of hydrocarbons under anoxic conditions has been investigated only during the past ten years. The present paper summarizes investigations into the anaerobic degradability of crude ...

Alkaline pre-treatment of lignocellulose is commonly used in bioethanol production and might also enhance the anaerobic digestion of lignocellulosic biomass to biogas. However, the elevated pH of the substrate may require an alkalitolerant microbial community for its effective digestion. Therefore, the aim of the present study was to enrich anaerobic, lignocellulolytic cultures batchwise from s...