The aim of this work was to identify enzymes that participate in the degradation of transitory starch in Arabidopsis. A mutant line was isolated by screening leaves at the end of the night for the presence of starch. The mutant had a higher starch content than the wild-type throughout the diurnal cycle. This accumulation was due to a reduction in starch breakdown, leading to an imbalance betwee...

background: today a large number of bacterial amylases are available commercially in industry. the goal of the present study was purification and biochemical characterization of an extracellular thermostable alkaline α-amylase from the novel moderately halophilic, bacillus persicus from the aran-bidgol, iran.   methods : purification of enzyme, was carried out by acetone precipitation, ultrafil...

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. the management of the blood glucose level is a critical strategy in the control of diabetes complications. inhibitors of carbohydrate hydrolyzing enzymes have been useful as oral hypoglycemic drugs for the control of hyperglycemia especially in patients with type ii disables mellitus. the goal of the present study...

Alpha-amylase inhibitor protein, which inhibits the activity of insect (Callosobruchus analis) α-amylase, was characterized from V. sublobata. The molecular weight of purified inhibitor protein was 14 kDa by SDS-PAGE. The inhibitor is non-glycosylated protein and its N-terminal sequence is similar (A P S P V...) to Phaseolus vulgaris α-AI-1. Its pI value is 6.0 and largely localised in cotyledo...

The cloning of a-amylase gene of S . occidentalis and the construction of starch digestible strain of yeast, S . cerevisiae AS. 2.1364 with ethanol-tolerance and without auxotrophic markers used in fermentation industry were studied. The yeast/E . coli shuttle plasmid YCEpl partial library of S. occidentalis DNA was constructed and a-amylase gene was screened in S. cerevisiae by amylolytic acti...

?-amylase inhibitory activities of ethanol extracts from six selected iranian salvia species (s. hydrangea dc., s. hypoleuca benth., s. officinalis l., s. reuterana boiss., s. verticillata l. and s. virgata jacq.) were examined, using in vitro model. in addition, antioxidant capacities (dpph• radical scavenging) and total flavonoid cotents of the extracts were also determined. the results showe...

A thermostable and detergent-stable α-amylase from a newly isolated Anoxybacillus sp. AH1 was purified and characterized. Maximum enzyme production (1874.8 U/mL) was obtained at 24 h of incubation. The amylase was purified by using Sephadex G-75 gel filtration, after which an 18-fold increase in specific activity and a yield of 9% were achieved. The molecular mass of the purified enzyme was est...

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

An extracellular α-amylase from the obligate halophilic Aspergillus penicillioides TISTR3639 strain was produced and enriched to apparent homogeneity by ammonium sulfate precipitation and Sephadex G100 gel filtration column chromatography. The mass of the purified amylase was estimated to be 42 kDa by SDS-PAGE. With soluble starch as the substrate it had a specific activity of 118.42 U · mg(-1)...