Degenerate primers-based polymerase chain reaction (PCR) are commonly used for isolation of unidentified gene sequences in related organisms. For designing the degenerate primers, we propose the use of local alignment search method for searching the conserved regions long enough to design an acceptable primer pair. To test this method, a WD40 repeat-containing domain protein from Beauveria bass...

p. atlantica subsp. kurdica, with the local name of baneh, is a wild medicinal plant which grows in kurdistan, iran.  the identification of resistance gene analogs holds great promise for the development of resistant cultivars. a pcr approach with degenerate primers designed according to conserved nbs-lrr (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (r) gene...

Background: Stearoyl-CoA desaturase (SCD) is a key enzyme that converts saturated fatty acids (SFAs) to monounsaturated fatty acids (MUFAs) in fat biosynthesis. Despite being crucial for interpreting SCDs’ roles across species, the evolutionary relationship of SCD proteins across species has yet to be elucidated. This study aims to present this evolutionary relationship based on amino aci...

prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. in the present investigation we report sequence of cdna encoding goat ( capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. as bovine prochymosin, the caprine prochymosin cdna encodes 365 amino ...

Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide and the pathogenicity of Newcastle disease virus (NDV) strains varies with different virulence. Samples were collected from chicken commercial farms in Iran during 2014–2015. ND virus were characterized (NDV) by partial sequences...

background: stearoyl-coa desaturase (scd) is a key enzyme that converts saturated fatty acids (sfas) to monounsaturated fatty acids (mufas) in fat biosynthesis. despite being crucial for interpreting scds’ roles across species, the evolutionary relationship of scd proteins across species has yet to be elucidated. this study aims to present this evolutionary relationship based on amino acid sequ...

The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysisof 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K codingregions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotyp...

objectives analysis of ns5a polymorphisms in hcv genotype 1b pre-treatment serum samples from estonian patients and their effect on the treatment response. patients and methods twenty-nine complete ns5a sequences obtained from patients with chronic hcv-1b infection who had received combined therapy with pegifnα-2a/rbv were analyzed and compared with the prototype strain hcv-j. twelve patients a...

This paper considers the generation of some interpretable fuzzy rules for assigning an amino acid sequence into the appropriate protein superfamily. Since the main objective of this classifier is the interpretability of rules, we have used the distribution of amino acids in the sequences of proteins as features. These features are the occurrence probabilities of six exchange groups in the seque...