A rapid procedure for the identification of Paenibacillus larvae subsp. larvae, the causal agent of American foulbrood (AFB) disease of honeybees (Apis mellifera L.), based on PCR and restriction fragment analysis of the 16S rRNA genes (rDNA) is described. Eighty-six bacterial strains belonging to 39 species of the genera Paenibacillus, Bacillus, Brevibacillus, and Virgibacillus were characteri...

The Gram-positive, spore-forming bacterium Paenibacillus larvae (P. larvae) is the causative agent of the epizootic American Foulbrood (AFB), a fatal brood disease of the western honey bee (Apis mellifera). AFB is one of the most destructive honey bee diseases since it is not only lethal for infected larvae but also for the diseased colonies. Due to the high impact of honey bees on ecology and ...

american foulbrood disease (afb) is one of the most important bacterial diseases of honey bee can be caused severe losses in apiaries. afb caused by paenibacillus larvae a gram positive and spore-forming bacterium. this study was taken placed in 15 area of east azerbaijan province of iran between 2006-2007 years. in this study, 500 bee hives were randomly selected from 100 apiaries. standard ba...

Considerable attention has been given to the effect of heating in various media on the viability and virulence of the spores of Bacillus larvae, the causative agent of the honeybee disease, American foul brood (White, 1920). Few data have been reported, however, concerning temperatures above the boiling point of honey (White, 1920; Burnside, 1940a, 1945). Under a project on sterilization of hon...

Paenibacillus larvae is a deadly pathogen for bee brood, which can lead to the death of entire colonies. The presence specific volatile organic compounds (VOCs) in hive may be related occurrence this bacterium brood. Compositions those fractions present healthy brood from control colonies and without symptoms infection collected infected by P. were compared using gas chromatography coupled with...

mojtaba moharami 1 - hossein modirrousta 2       1-assistant professor of  bee pathology, razi vaccine and serum research institute. 2- msc of  microbiology, razi vaccine and serum research institute abstract  american foulbrood (afb), a severe bacterial disease of honeybee brood, caused by the sporeforming bacterium paenibacillus larvae subspecies larvae. the agent, infect and kill larvae and ...

AIM To develop a real-time PCR-based strategy for the detection of Paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of American foulbrood (AFB), the most harmful pathology of honeybee brood. METHODS AND RESULTS A real-time PCR that allowed selective identification and quantification of P. larvae 16S rRNA sequence was developed. Using standard samples...

Paenibacillus larvae is a Gram positive bacterium and the causative agent of the most widespread fatal brood disease of honey bees, American foulbrood (AFB). A total of thirty-three independent Paenibacillus larvae isolates from various geographical origins in North America and five reference strains were investigated for genetic diversity using multilocus sequence typing (MLST). This technique...

The microorganisms Ascosphaera apis, Paenibacillus larvae and Melissococcus plutonius are the three most important pathogens that affect honeybee brood. The aim of the present study was to evaluate the prevalence of these pathogens in honeybee colonies and to elucidate their role in the honeybee colony losses in Spain. In order to get it, a multiplex polymerase chain reaction (PCR) assay was de...