A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavele...

The peroxidase of human parotid saliva has been purified by concentration, gel filtration on Sephadex G-200, and ion exchange chromatography on Amberlite CG-50. The purified product was devoid of amylase activity, lysozyme activity, and immunoglobulin A (IgA). However, it had an inhibitory effect on the growth of Lactobacillus acidophilus in complete growth medium and on lysine accumulation by ...

I. A unique hemoprotein has been isolated from a hemolysate of normal human erythrocytes by a procedure which involves (NH4),SO 4 precipitation and chromatography on Amberlite CG-5o, DEAE-cellulose, Bio-Gel P-6o, and Bio-Gel P-3o. 2. The purified hemoprotein appeared homogeneous on ultracentrifugation although small amounts of impurities were detected by polyacrylamide gel electrophoresis. The ...

A clinically useful spectrophotometric method for the determination of 3-methoxy-4hydroxyphenyiglycol (MHPC) is presented. After treatment with barium chloride, the urine is hydrolyzed with Glusulase ($-glucuronidase and sulfatase).f The hydrolysate is passed through a column of Amberlite CG-4Bt to remove any 3methoxy-4-hydroxymandelic acid (MHMA) and the pH is adjusted to neutral. The MHPG is ...

During an initial search for pharmacologically active principles in red-mold rice, two hypotensive compounds were isolated and identified. The active principles, A-3 and B-1, were fractionated and purified from the EtOH extract of red-mold rice prepared with Monascus pilosus through extraction with H2O, Amberlite CG-120 column chromatographies and high-performance liquid chromatography on an OD...

Raw-starch-digesting enzyme (RSDA) was immobilized on Amberlite beads by conjugation of glutaraldehyde/ polyglutaraldehyde (PG)-activated beads or by crosslinking. The effect of immobilization on enzyme stability and catalytic efficiency was evaluated. Immobilization conditions greatly influenced the immobilization efficiency. Optimum pH values shifted from pH 5 to 6 for spontaneous crosslinkin...

We evaluated the competitive fitness of three phenanthrene-mineralizing isolates that were provided with nonsorbed or sorbed phenanthrene in competition experiments. One isolate had a clear advantage when presented with Amberlite IRC-50-sorbed phenanthrene, yet none were favored when presented with nonsorbed or Amberlite XAD-7-sorbed phenanthrene. Our results indicate that contaminant availabil...

A new member of paromomycin group antibiotics has been isolated from the culture filtrate of Streptoverticillium sp. Al-R2827 which was isolated from a soil sample collected in India. In this report, the production, the isolation, characterization and structural elucidation of the antibiotic are reported. Production and Isolation Streptoverticillium sp. Al-R2827 was inocu-lated into a seed medi...

This work studies the feasibility of the use of a combined physical-biological remediation procedure for treatment of effluents contaminated with molinate, where the herbicide is removed through adsorption and biodegraded in a subsequent stage, with the regeneration of the adsorbent. In order to select the most adequate absorbent for molinate, different materials were tested, namely pine bark, ...

Uranium (VI) sorption using an anionic exchanger resin in the presence of sulfate anions, namely amberlite 910, was the subject of current study. Batch sorption experiments were carried out to evaluate the influence of operational parameters such as pH, contact time, initial concentration and existence of various anions (including phosphate, sulfate, chloride, fluoride, and nitrate) in the solu...