The principal advantage of autologous blood transfusions is the danger of transmitting infectious agents and the adverse immunomodulatory effects of allogenic transfusion can be avoided. The effectiveness of preoperative autologous blood donation in reducing allogenic blood exposure is undisputed. The current discussion focuses on cost effectiveness versus the potential risks of autologous bloo...

OBJECTIVE To study the feasibility of engineering cartilage tissue in a tube lined with epithelium and implanting allogenic chondrocytes in a novel scaffold that is made of chitosan nonwoven cloth coated with poly(DL-lactide-co-glycolide). DESIGN Laboratory research. SUBJECTS Allogenic chondrocytes were obtained from the auricles of 1-month-old New Zealand white rabbits. After the cells wer...

BACKGROUND We sought to determine the effectiveness of chondroprogenitor cells derived from autologous and allogenic articular cartilage for the repair of cartilage defects in an equine model. METHODS Cartilage defects (15 mm) were created on the medial trochlear ridge of the femur. The following experimental treatments were compared with empty-defect controls: fibrin only, autologous chondro...

The effect of intraoperative cell salvage (ICS) in surgical correction of spinal deformity remained controversial. This study was to quantitatively demonstrate its effect. In all, 124 patients having ICS in surgical correction of spinal deformity were included. These patients would be divided into 3 groups. Group 1-blood loss less than 15 mL/kg; group 2-between 15 and 37.5 mL/kg; and group 3-mo...

We studied the use of autologous pre-donatedblood transfusion in surgery for scoliosis in 45 patients who were divided into two groups; 27 who pre-donated autologous blood (group 1) and 18 who were planned recipients of allogenic blood (group 2). Normovolaemic haemodilution and intra-operative blood salvage was used in six patients in group 1 and three patients in group 2. The two groups did no...

Karst aquifer studies often focus on allogenic water inputs and large conduit flow. However, diffuse recharge can be significant, particularly in unconfined eogenetic karst aquifers that retain high matrix permeability. This study examines an unconfined region of the upper Floridan aquifer (USA) that hosts a sinking stream, its resurgence, and a large conduit system. Daily diffuse recharge was ...

OBJECTIVE Massive bone allografts are frequently used in orthopedic reconstructive surgery, but carry a high failure rate of approximately 25%. We tested whether treatment of graft with mesenchymal stem cells (MSCs) can increase the integration of massive allografts (hemi-mandible) in a large animal model. METHODS Thirty beagle dogs received surgical left-sided hemi-mandibular defects, and th...

Allogenic blood is a finite resource, with associated risks. Previous studies show intraoperative cell salvage (ICS) can reduce allogenic transfusion rates in orthopaedic surgery. However, there are concerns regarding efficacy and cost-effectiveness of ICS. This study was carried out to review ICS use in revision hip arthroplasty. All patients who underwent ICS and re-infusion between 2008 and ...

Allogeneic bone marrow transplantation (BMT) after high-dose, marrow-ablative chemoradiotherapy has been established as the treatment of choice for various hematologic, neoplastic, and congenital disorders. The most common type of marrow graft is an allogeneic one from a sibling donor who has compatible human leukocyte antigen (HLA). Only 30% of patients requiring allogeneic BMT have an HLA-com...

Background: Dendritic cells (DCs) are professional antigen presenting cells (APCs), and there is considerable interest in their application as a cellular adjuvant for cancer immunotherapy.  Previous studies indirectly demonstrated that DCs were able to take up tumor lysate (crude soluble tumor antigens) and also cross-present tumor associated antigens (TAA) which elicits anti-tumor immune respo...