The allergenic potency of spore and mycelium extracts of Cladosporium was estimated by RAST, RAST inhibition and PCA tests. Spores contained a concentration of allergens higher than mycelia. Results of PCA tests suggested that spores contained specific allergens. However, in a comparative study of extracts from different species of Cladosporium animal and human models gave different estimates o...

Serum IgE assay is a mainstay step in the allergy work up. Allergenic extracts and molecular components are available at present. This real life study compared the serum specific IgE levels against allergenic extracts with allergenic molecules in patients allergic to Parietaria, Betulaceae, and mites. This retrospective real life study included 489 subjects with respiratory allergy. Inclusion c...

Sesquiterpene lactones (SLs), mainly those with an activated exocyclic methylene group, are important allergens in Asteraceae (Compositae) plants. As a screening tool, the Compositae mix, consisting of five Asteraceae plant extracts with allergenic potential (feverfew, tansy, arnica, yarrow, and German chamomile) is part of several national patch test baseline series. However, the SL content of...

Results After 80% ammonium sulphate precipitation, most of the proteins were recovered in 80% precitable fraction, 58.4% in moth and 71.2% in rice weevil. However, the recovery in supernatant was low 6.1% in rice weevil and 14.4 in moth extract. In ELISA inhibition of both the insect WBEs, most of the allergenic activity was recovered in 80% ammonium sulphate precipitate fraction (80% ppt). No ...

BACKGROUND Skin tests for specific diagnosis of allergies require high quality extracts, which can be measured by the determination of allergenic potency, utilizing different concentrations of extracts from a manufacturer. OBJETIVE To compare allergenic mite extracts manufactured by laboratories in Spain, Argentina, and Cuba by skin prick test response. METHODS A cutaneous puncture was perf...

Relevant allergens are major contributors to the safety and efficacy of allergenic extracts used in allergen immunotherapy (AIT). As such, they should be accurately quantified, as recommended by the 2008 European guidelines on allergen products. Until now, the quantification of relevant allergens was mainly performed by using immunoassays (e.g. ELISA) that relying upon specific antibodies. Alth...

the allergoid (P 1⁄4 0.16; Mann-Whitney). Linear regression coefficients obtained between immunoglobulin reactivity against both extracts were: r2 1⁄4 0.51 for specific IgE and r2 1⁄4 0.83 for specific IgG. An important decrease in the allergenic activity, measured by inhibition ELISA, was clearly observed. The MS/MS assay revealed the presence of the mayor allergen, and some isoforms, in non-m...

the allergoid (P 1⁄4 0.16; Mann-Whitney). Linear regression coefficients obtained between immunoglobulin reactivity against both extracts were: r2 1⁄4 0.51 for specific IgE and r2 1⁄4 0.83 for specific IgG. An important decrease in the allergenic activity, measured by inhibition ELISA, was clearly observed. The MS/MS assay revealed the presence of the mayor allergen, and some isoforms, in non-m...

m wild plants, especially when travelling abroad. The first step in acquiring such an interest is to procure a book describing the flowers to be found in some place where one is going for a holiday. Such a one is " Flowers of the Mediterranean " by Polunin and Huxley. I choose this because so many people make for that part of Europe and it is a wonderful area in which to start. Armed with such ...

This study analyzes the exchange rate pass-through into China aggregate import prices and prices of eleven industries under the HS classification. Chinese monthly data from July 2005 to July 2015 are used. Bounds test finds that all industries have co-integration relationship, with the exception of footwear and headgear products (HS12) and transport equipment (HS17). We have researched the shor...