Organisms growing aerobically generate reactive oxygen species such as hydrogen peroxide. These reactive oxygen molecules damage enzymes and DNA, potentially causing cell death. In response, Bacillus subtilis produces at least nine potential peroxide-scavenging enzymes; two belong to the alkylhydroperoxide reductase (Ahp) class of peroxidases. Here, we explore the role of one of these Ahp homol...

The Mycobacterium tuberculosis ahpC gene, encoding the mycobacterial orthologue of alkylhydroperoxide reductase, undergoes an unusual regulatory cycle. The levels of AhpC alternate between stages of expression silencing in virulent strains grown as aerated cultures, secondary to a natural loss of the regulatory oxyR function in all strains of the tubercle bacillus, and expression activation in ...

alkylhydroperoxide reductase (ahpc, the 26 kda antigen) is one of the abundant antioxidant enzymes in helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect h. pylori infection in clinical specimens. this study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. five established hybridoma cell lines secreti...

Alkylhydroperoxide reductase (AhpC, the 26 kDa antigen) is one of the abundant antioxidant enzymes in Helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect H. pylori infection in clinical specimens. This study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. Five established hybridoma cell lines secreti...

The synthesis and characterization of new molecular sieves of the MCM-41 type are presented in this study. Samples were obtained from the alkoxides of Si, Ti and Al and different long-chain amine and alkylammonium salts surfactants, by the sol-gel method, through moderate agitation at room temperature. The reactions were conducted in two steps, acid hydrolysis (pH=2) and alkaline condensation (...

Streptococcus pyogenes strains can be divided into two classes, one capable and the other incapable of producing H2O2 (M. Saito, S. Ohga, M. Endoh, H. Nakayama, Y. Mizunoe, T. Hara, and S. Yoshida, Microbiology 147:2469-2477, 2001). In the present study, this dichotomy was shown to parallel the presence or absence of H2O2-producing lactate oxidase activity in permeabilized cells. Both lactate o...

materials and methods total protein was determined by lowery method. bovine lens alr2 was partially purified by gel filtration chromatography on sephadextm g25. bovine cortex kidney alr1 was partially purified by diethylaminoethyl (deae) precipitation. the hydroalcoholic extract was obtained from frozen propolis. the enzyme activity and sorbitol accumulation in erythrocytes were determined spec...