A combination of steam explosion (SE) and alkaline peroxide (AP) used to pretreat bamboo was investigated. Steam explosion at 224 °C for 4 min was applied to bamboo, and the pretreated bamboo was delignified by alkaline peroxide. Enzymatic hydrolysis was compared in the pretreated samples. Steam pretreatment led to remarkable hemicellulose solubilization (63.2%). Lignin solubilization (93.1%) w...

Sludge fermentation between pH 4 and 11 was investigated to generate volatile fatty acids (VFA). Despite the highest sludge solubilization of 25.9% at pH 11, VFA accumulation was optimized at pH 8 (12.5% out of 13.1% sludge solubilization). 454 pyrosequencing identified wide diversity of acidogens in bioreactors operated at the various pHs, with Tissierella, Petrimonas, Proteiniphilum, Leviline...

Thermo-chemical pre-treatment improves the solubilization of the lignocellulosic biomass. Therefore, it could improve biogas production from press mud, the solid residue from sugar cane juice filtration which contains up to 85 % insoluble organic matter. The impact of thermo-alkaline pretreatment conditions (100 o C, Ca(OH)2) on press mud was investigated for different times and concentrations,...

The alkaline phosphatase activities of 6-thiopurine-sensitive and -resistant sublines of Sarcoma 180 were exam ined in a further study of these enzymes, which have a role in the resistance of Sarcoma 180/TG to 6-thioguanine and 6-mercaptopurine. Both neoplasms possessed particulate-bound alkaline phosphatase enzymes labeled A and B on the basis of their ease of solubilization from the particula...

Canine microsomal signal peptidase activity was previously isolated as a complex of five subunits (25, 22/23, 21, 18, and 12 kDa). Two of the signal peptidase complex (SPC) subunits (23/23 and 21 kDa) have been cloned and sequenced. One of these, the 21-kDa subunit, was observed to be a mammalian homolog of SEC11 protein (Sec11p) (Greenburg, G., Shelness, G. S., and Blobel, G. (1989) J. Biol. C...

background and objectives: azotobacter is a diazotroph bacterium reported to possess various plant growth-promoting characteristics.the aim of this study was to isolate azotobacter strains capable of fixing nitrogen and effectively hydrolyzing both organic and inorganic pi compounds. materials and methods: in this study, soil samples collected from a diverse range of slightly alkaline soil type...

Inclusion bodies produced in Escherichia coli are composed of densely packed denatured protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive forms is cumbersome, results in poor recovery and accounts for the major cost in production of recombinant proteins from E. coli. With new information available on the structure and function of protein aggregates i...

1. Incubation of Schistosoma mansoni for 5 min in a phosphate-buffered medium, pH 7.4, released tegumental material containing the following phosphohydrolase activities: alkaline phosphatase, 5'-nucleotidase, glycerol-2-phosphatase, glucose 6-phosphatase, phosphodiesterase and ATPase. 2. Maximum activity of these enzymes was measured at pH 9.5; however, the phosphodiesterase and ATPase activiti...