background: hydatid cysts are formed in human body can be treated clinically by surgery or drugs such as albendazole (abz) and mebendazole (mbz). the purpose of this study was comparing the effects of abz and mbz on glutathione-s-transferase, alkaline phosphatase and protease enzymes activities in protoscoleces of hydatid cyst. methods: the culture supernatants containing the parasite excretory...

The purpose of this study is to improve the thermo-stabilty of alkaline protease produced by Bacillus sp. using rice bran during solid state fermentation. After partial purification of alkaline protease, various parameters namely effect of temperature and metal ions on enzyme activity was investigated. The suitable temperature for alkaline protease activity was observed at 50°C. Among various m...

Background: Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives: The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi.<br...

Alkaline proteases have biotechnological importance due to their activity and stability at alkaline pH. 56 bacteria, capable of growing under alkaline conditions were isolated and their alkaline protease activities were carried out at different parameters to determine their optimum alkaline protease production conditions. Seven isolates were showed higher alkaline protease production capacity t...

alkaline crude enzymes from the viscera of the tunisian barbel (barbus callensis) were extracted and characterized. proteolytic crude extract from barbel viscera was active and stable in alkaline solution. the optimum ph and temperature were 11.0 and 55 °c, respectively, using casein as a substrate. the crude alkaline protease was extremely stable in the ph range of 5.0-12.0. zymography activit...

The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...

A highly sensitive and specific radioimmunoassay has been developed for Pseudomonas aeruginosa alkaline protease. Production of alkaline protease was found to be strain variable and medium dependent.

Alkaline crude enzymes from the viscera of the Tunisian barbel (Barbus callensis) were extracted and characterized. Proteolytic crude extract from barbel viscera was active and stable in alkaline solution. The optimum pH and temperature were 11.0 and 55 °C, respectively, using casein as a substrate. The crude alkaline protease was extremely stable in the pH range of 5.0-12.0. Zymography activit...

Alkaline crude enzymes from the viscera of the Tunisian barbel (Barbus callensis) were extracted and characterized. Proteolytic crude extract from barbel viscera was active and stable in alkaline solution. The optimum pH and temperature were 11.0 and 55 °C, respectively, using casein as a substrate. The crude alkaline protease was extremely stable in the pH range of 5.0-12.0. Zymography activit...

Proteases, one among the three largest groups of industrial enzymes, accounts for about 60% of the total worldwide sale of enzymes from biological sources since they possess almost all characteristics desired for their biotechnological applications (Adinarayana et al., 2003). Among the various proteases, bacterial protease was the most significant compared with animal, fungi and plant protease....