Chronic lung infection with mucoid, alginate-producing strains of Pseudomonas aeruginosa is a major cause of mortality in cystic fibrosis (CF) patients. Transcriptional activation of the P. aeruginosa algD gene, which encodes GDPmannose dehydrogenase, is essential for alginate synthesis. Activation of algD is dependent on the product of the algR gene. Sequence homology between the P. aeruginosa...

The type III secretion system (T3SS) of Pseudomonas aeruginosa is an important virulence factor. The T3SS of P. aeruginosa can be induced by a low calcium signal or upon direct contact with the host cells. The exact pathway of signal sensing and T3SS activation is not clear. By screening a transposon insertion mutant library of the PAK strain, mutation in the mucA gene was found to cause repres...

H(2)O(2) production and changes in glutathione, catalase, and peroxidase were followed in whole-leaf extracts from the susceptible (AlgS [Algerian/4* (F14) Man.(S)]; ml-a1 allele) and resistant (AlgR [Algerian/4* (F14) Man.(R)]; Ml-a1 allele) barley (Hordeum vulgare) isolines between 12 and 24 h after inoculation with powdery mildew (Blumeria graminis [DC]. Speer [syn. Erysiphe graminis DC] f.s...

Many respiratory isolates of Pseudomonas aeruginosa from cystic fibrosis patients are mucoid (alginate producing) yet lack flagella. It was hypothesized that an alginate regulator inhibits flagellar gene expression. Mutations in algB, algR, and algT resulted in nonmucoid derivatives, yet algT mutants expressed flagella. AlgT-dependent control of flagellum synthesis occurred through inhibition o...


 Значне поширення резистентних штамів P. aeruginosa, здатність до плівкоутворення, складні регуляторні мережі формування біоплівок і багаточисельні фактори вірулентності потребують пошуку нових сполук розробки препаратів, які здатні порушувати біоплівок.
 Мета дослідження – вивчити експресію генів, що регулюють біоплівки aeruginosa за дії похідного адамантану.
 Антибіоплівкову а...

The Gram-negative opportunistic pathogen Pseudomonas aeruginosa ubiquitously inhabits soil and water habitats and also causes serious, often antibiotic resistant, infections in immunocompromised patients (e.g. cystic fibrosis). This versatility is mediated in part by a large repertoire of two-component regulatory systems that appear instrumental in the regulation of both virulence processes and...

in this study, ultrafine grained 2024 al alloy based b4c particles reinforced composite was produced by mechanical milling and hot extrusion. mechanical milling was used to synthesize the nanostructured al2024 in attrition mill under argon atmosphere up to 50h. a similar process was used to produce al2024-5%wt. b4c composite powder. to produce trimodal composites, milled powders were combined w...