To design an enzyme immunoassay-based method, the most important is conjugation of enzyme with antigens or antibodies and stability of labeled conjugate. In this study, human albumin was conjugated with horseradish peroxidase using periodate oxidation method. To stabilize biological activity and functional structure of different components of protein conjugate, special storage conditions are re...

Purified peptidyl dipeptidase (angiotensin I converting enzyme or kininase II) from human lung or hog kidney is inhibited by commercially prepared plasma protein preparations, by human serum albumin and by the additive albumin stabilizer, acetyltryptophan. After the initial steps of purification, albumin was detected by immunodiffusion as a component in human lung peptidyl dipeptidase preparati...

A method of tethering a mediator to an enzymatic membrane was studied. Glucose oxidase (GOD) and ferrocene redox polymer were immobilized in cross-linked poly (vinyl alcohol) (CLPVA) with bovine serum albumin (BSA) added as a protein stabilizer. Redox hydrogel polyallylamine ferrocene was prepared by cross-linking polyallylamine hydrochloride with glutaraldehyde and attaching the ferrocene cova...

Sodium octanoate and N-acetyl-L-tryptophan (N-AcTrp) are widely used as stabilizers during pasteurization and storage of albumin products. However, as compared with N-AcTrp, N-acetyl-L-methionine (N-AcMet) is superior in protecting albumin exposed to light during storage. Here, we examine, whether N-AcMet also is better than N-AcTrp to protect albumin against oxidation. Recombinant human serum ...

Creatine kinase (CK; EC 2.7.3.2) has been used as an indicator of myocardial cellular damage. In this study we used a Krebs-Henseleit (KH) solution to reperfuse isolated rat hearts after 24 h of cold preservation and collected the KH reperfusate for assay of CK to assess cellular damage. We wanted to determine the stability of CK in the KH solution at different cold-storage temperatures and alb...

In this study, the effect of basil seed gum (BSG) on the stability and rheological properties of egg white albumin (EWA) emulsions was investigated. A constant amount of EWA (0.5% w/v) and different concentrations of BSG (0-0.3% w/v) were used for the manufacture and stabilization of oil-in-water emulsions (30% w/w). The results showed that by increasing BSG concentration, the storage (21 days)...

Large-scale quantum computation is likely to require massive quantum error correction (QEC). QEC codes and circuits are described via the stabilizer formalism, which represents stabilizer states by keeping track of the operators that preserve them. Such states are obtained by stabilizer circuits (consisting of CNOT, Hadamard and Phase gates) and can be represented compactly on conventional comp...

Powder aerosols made of albumin, dipalmitoylphosphatidylcholine (DPPC) and a protein stabilizer (lactose, trehalose or mannitol) were prepared by spray-drying and analyzed for aerodynamic behavior, surface composition and physical state. The powders exited a Spinhaler inhaler as particle aggregates, the size of which depending on composition, spray-drying parameters and airflow rate. However, d...

Human plasma has not gained general acceptance as a therapeutic agent because of the risk of hepatitis following its use. A number of physical and chemical treatments have been tried to minimize this risk but the incidence remains about 0.5 per cent for single donor plasma and upwards of 20 per cent for some lots of pooled plasma (1, 2). Ultraviolet irradiation, which at one time seemed quite p...