Linum spp. (Linaceae) has been shown to accumulate cytotoxic lignans such as podophyllotoxin and 6-methoxypodopyllotoxin. Therefore, plant cell and tissue cultures of Linum spp. may be possible alternative sources for the lignan production. In this study, we focused on the production of lignans by root cultures of Linum spp. Hairy root cultures of L. nodiflorum, L.album and L. austriacum and ro...

Rhizobium, Agrobacterium and Allorhizobium are genera within the bacterial family Rhizobiaceae, together with Sinorhizobium. The species of Agrobacterium, Agrobacterium tumefaciens (syn. Agrobacterium radiobacter), Agrobacterium rhizogenes, Agrobacterium rubi and Agrobacterium vitis, together with Allorhizobium undicola, form a monophyletic group with all Rhizobium species, based on comparative...

organogenesis and neoformant plantlet production were studied by culturing the different parts of mature embryo including radicle and proximal and distal parts of cotyledon of mission and kroneiki cultivars. different parts of embryos were cultures in omc medium containing growth regulators, such as indole butyric acid (iba) and 2-isopentenyl adenine (2-ip) or iba and naphthalene acetic acid (n...

This work describes a micropropagation protocol for plants of Cynara scolymus cv. Early French, employing cyclodextrins during the rhizogenesis stage. Shoot apices were established in basic culture medium consisting of Murashige Skoog mineral salts modified by 50% reduction in NH4NO3 and KNO3, Heller's micronutrients, Gamborg's vitamins, 100 mg l ÿ1 myo-inositol, 30 g lÿ1 saccharose and 8.0 g l...

Agrobacterium: Microbe Wiki http://microbewiki.kenyon.edu/index.php/Agrobacterium This website provides an excellent overview of the genus Agrobacterium. It contains photos and links to additional information. Genome of Agrobacterium tumefaciens http://www.genomenewsnetwork.org/articles/12_01/ A_tumefaciens_genome.shtml A. tumefaciens has been the most widely studied and used Agrobacterium spec...

Non-nodulating Agrobacterium-like strains identified among root nodule isolates of common bean were labeled with gusA, a reporter gene encoding beta-glucuronidase (GUS). Bean plants were then co-inoculated with an infective Rhizobium strain and labeled transconjugants of Agrobacterium-like strains. Blue staining of nodules showed that Agrobacterium-like strains were able to colonize these symbi...

As a special phytopathogen, Agrobacterium tumefaciens infects a wide range of plant hosts and causes plant tumors also known as crown galls. The complexity of Agrobacterium-plant interaction has been studied for several decades. Agrobacterium pathogenicity is largely attributed to its evolved capabilities of precise recognition and response to plant-derived chemical signals. Agrobacterium perce...

Azotobacter vinelandii stimulated the growth of Agrobacterium tumefaciens H2, H23, H24, H27, and ATCC 15955 on media containing insoluble iron sources. The Azotobacter vinelandii siderophores appeared to promote Agrobacterium tumefaciens growth by solubilizing mineral iron, and the ferrisiderophores so formed then acted as iron sources for Agrobacterium tumefaciens. Agrobactin, the Agrobacteriu...