INTRODUCTION Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis. METHODOLOGY Peripheral blood samples from 34 suspected brucellosis patients and 42 probable chronic o...

Similar results were obtained when testing human sera for Salmonella antibodies by the tube agglutination test and by the Microtiter plate agglutination test. The plate test was easier to perform and saved time, space, antigen, and serum.

A comparison is made of tests for thyroglobulin antibody, using gel diffusion, electroprecipitin, bentonite flocculation, tanned red cell agglutination, and latex slide agglutination techniques on sera from cases of Hashimoto's disease and other thyroid disorders. Any increase in gamma globulin was also noted from the serum electrophoretic pattern. The gel diffusion and electroprecipitin tests ...

Recent research into the nature and significance of rheumatoid serum factors has been largely immunological and biochemical. The epidemiology of these components is virtually unknown. During recent years we have had the opportunity of studying several population samples, and data from these surveys have now been gathered and are presented with particular reference to the epidemiology of the ser...

Fifty sera collected from 12 different species of free-living game animals in game parks in the Northern Natal were tested against 8 Leptospira interrogans antigens using the microscopic agglutination test (MAT). Six out of 50 animals had titres, all less than 100. Three of these animals had titres to serovar mini, 1 animal to tarrasovi, and 3 animals had multi-serovar reactions, 1 to mini and ...

We review the significance of the Widal agglutination test in the diagnosis of typhoid fever. Over 100 years since its introduction as a serologic means of detecting the presence of typhoid fever, the Widal test continues to be plagued with controversies involving the quality of the antigens used and interpretation of the result, particularly in endemic areas. Areas of concern with clinical and...

AIMS To compare the established Widal agglutination assay with an immunoblotting procedure. METHODS 110 sera were used to compare the established Widal agglutination assay with an immunoblotting procedure incorporating lipopolysaccharide (LPS) (O = 9,12) and flagellar (H = d) antigens. RESULTS Antibodies to the LPS antigens were detected in 18 sera by the Widal assay and in 37 by immunoblot...