Antibodies were prepared against carboxydismutase and latent Ca2® dependent ATPase (coupling factor) purified from Nicotiana tabacum. The antibodies against carboxydismutase inhi­ bit the enzymatic activity of the purified protein, while those against coupling factor inhibit the Ca2© dependent ATPase activity of the protein as well as the photophosphorylation of the chloro­ plasts. The antisera...

The optical properties of lunar softs are different than those of rocks from which they are derived. As a consequence of lunar space weathering, soils are darker and exhibit a distinctive red-sloped continuum and weaker mineral absorption bands. The accumulation of dark glass-welded aggregates (agglutinates) has been thought to account for these optical effects of space weathering on lunar soil...

Pure spore antigens for the immunization of rabbits were prepared by enzymic digestion of vegetative components and separation of the cleaned spores in polyethylene glycol. Spore antisera were prepared to strains representative of toxigenic Clostridium botulinum type E; nontoxigenic boticin E-producing variants; nontoxigenic nonproducers of boticin E; nontoxigenic "atypical" strains, which diff...

Serum (antigen) when heated at a temperature sufficient to cause definite clouding reacts more intensely with a specific precipitin than a portion of the unheated serum or samples heated at lower temperatures. The phenomenon is explained on the basis that coagulated protein in suspension is covered with undenatured antigen and the addition of precipitin causes agglutination of the coagulated pr...

Introduction: Since the return of the first lunar samples it has been well known that glass-welded aggregates (agglutinates) accumulate in lunar soil as the result of multiple processes, many of which are driven by micrometeorite impacts. The proportion of agglutinates increases with increasing exposure to the space environment, and for an individual soil the proportion of agglutinates also inc...

Seventeen strains of ten different species of coagulase-negative staphylococci were shown to interact with collagen, laminin, fibronectin, and vitronectin immobilized on latex beads. Different species of coagulase-negative staphylococci have different capacities to agglutinate proteins. Cells of 18 strains of Staphylococcus haemolyticus reacted more strongly than did cells of 18 Staphylococcus ...

Most strains of animal rotaviruses are able to agglutinate erythrocytes, and the surface protein VP4 is the virus hemagglutinin. To map the hemagglutination domain on VP4 while preserving the conformation of the protein, we constructed full-length chimeras between the VP4 genes of hemagglutinating (YM) and nonhemagglutinating (KU) rotavirus strains. The parental and chimeric genes were expresse...

Two independently isolated mutant strains, imp-10 and imp-12, were obtained by UV irradiation of wild-type mating-type minus (wt(-)). Each fails to agglutinate sexually with gametes of either mating type, but mating and zygote formation can be elicited by agglutinating either strain to wt(+) gametes by means of anti-flagellar antiserum. Tetrad analysis of the resultant zygotes shows that both i...

Rabbit antibodies induced against alprenolol, a potent beta-adrenergic antagonist, bind to other antagonists, and, with less avidity, to catecholamine agonists. Anti-idiotypic antibodies were raised against the anti-alprenolol immunoglobulins. The anti-idiotypic antibodies specifically bind and agglutinate turkey erythrocytes; this is not observed for human agglutinate turkey erythrocytes; this...

Hybridomas secreting monoclonal antibodies specific for Pseudomonas aeruginosa outer membrane antigens were isolated. One of the antibodies was highly specific for the O antigen of the lipopolysaccharide of International Antigen Typing Scheme serotype 5 strains, reacting only weakly with a serotype 17 strain and failing to react with the outer membranes of strains representing 15 other serotype...