background: the microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. methods: in this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. for fabrication of these substrates, a thin film of ox...

Introduction: The PAGAT polymer gel dosimeter melts at 30 ˚C and even at room temperature during the summer, so it needs to be kept in a cool place such as a refrigerator. To increase the stability of the PAGAT gel, different amounts of agarose were added to the PAGAT gel composition and the PAGATA gel was manufactured. Material and Methods: The PAGATA gel vials were irradiated using a Co-60 ma...

Giant unilamellar vesicles (GUVs) are presumably the current most popular biomimetic membrane model. Preparation of GUVs in physiological conditions using the classical electroformation method is challenging. To circumvent these difficulties, a new method was recently reported, by which GUVs spontaneously swell from hybrid films of agarose and lipids. However, agarose is left encapsulated in th...

1. Chromatographic studies have been made of the affinities of a number of purified proteins for agarose (Sepharose 4B) substituted with 4-phenylbutylamine (PBA) or with E-aminocaproyl-n-tryptophan methyl ester (ACTME) as compared to controls of untreated agarose and/or of cyanogen bromide treated agarose without addition of a substituting amine. 2. At pH 8, cu-chymotrypsin (3.4.4.5) and 7s y-g...

Lipase from Thermomyces lanuginosus (TLL) has been immobilized on Purolite Lifetech® ECR8806F (viz. methacrylate macroporous resin containing octadecyl groups, designated as C18-TLL), and the enzyme performance compared to that of octyl-agarose, agarose C8-TLL. The hydrolytic activity versus p-nitrophenol butyrate decreased significantly, a lower extent S-methyl mandelate (more than twofold), w...

Hydroxyapatite (HA) or calcium carbonate (CaCO3) formed on an organic polymer of agarose gel is a biomaterial that can be used for bone tissue regeneration. However, in critical bone defects, the regeneration capability of these materials is limited. Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into bone forming osteoblasts. In this study, we loaded MSCs on HA- or ...

introduction: the pagat polymer gel dosimeter melts at 30 ˚c and even at room temperature during the summer, so it needs to be kept in a cool place such as a refrigerator. to increase the stability of the pagat gel, different amounts of agarose were added to the pagat gel composition and the pagata gel was manufactured. material and methods: the pagata gel vials were irradiated using a co-60 ma...

Physiologically, a hallmark of biological hydrogels is their ability to selectively trap diffusing molecules and particles. And indeed, there now increasing interest in using selective hydrogel barriers for applications biomedicine medical engineering. However, when employing synthetic polymers create with permeabilities, controlling the type strength ensuing filtration process difficult. Here,...