background: leishmaniases are a group of diseases caused by leishmania parasites .  growing of drug unresponsiveness in leishmaniasis patients necessitates the development of new drugs and accor­dingly a suitable assay is needed for evaluation of any modalities.  the aim of this study was to compare four drug assays methods, agar dilution, broth dilution, cylinder plate and disk diffusion, for ...

plants are potent biochemists; biologically active compounds present in the medicinal plants have always been ofgreat interest to scientists working in this field. thus, the aim of the current study was to screen the antimicrobial activity of acacia nilotica, ziziphus mauritiana, bauhinia variegate and lantana camara against some selected clinical isolated strains. although previous studies hav...

Background and Aim:  Streptococcus sanguinis (S. sanguinis) is one of the effective bacteria in the formation of caries and periodontal diseases which are caused by dental plaque. Currently, the use of natural honey (NH) is considered as a method of reducing S. sanguinis and S. mutans in saliva, which results in reducing the dental plaque. The purpose of this study was to evaluate the effects o...

Previously, we showed that microbial susceptibility to antimicrobials in concentrations non-toxic for human cells in culture could be tested using the wet disc topical antimicrobial assay. In this report, wet disc assay and agar well diffusion assay results were compared testing the susceptibility of Ps. aeruginosa isolates from burn patients to concentrations of Polymyxin B non-toxic for cultu...

A variety of devices such as stainless-steel or ceramic cylinders, single or multiple in design, and paper discs of several sizes are commonly used for antibiotic assays performed by the agar diffusion method. Holes cut in agar (agar wells) are wells with melted agar is often necessary to obtain uniform inhibition zones (2). This note describes a method for the preparation of agar-well plates w...

Different antimicrobial susceptibility testing methods to detect low-level vancomycin resistance in enterococci were evaluated in a Scandinavian multicenter study (n=28). A phenotypically and genotypically well-characterized diverse collection of Enterococcus faecalis (n=12) and Enterococcus faecium (n=18) strains with and without nonsusceptibility to vancomycin was examined blindly in Danish (...

The relative responses of neomycins B and C have been determined by a microbiological agar-diffusion method, a turbidimetric method, and by a recently developed gas-liquid-chromatographic (GLC) method capable of separating the neomycin isomers. The ratios of response of neomycin C to neomycin B by the individual methods were as follows: agar-diffusion method, 1:3; turbidimetric method, 1:2.5; a...

BACKGROUND Leishmaniases are a group of diseases caused by Leishmania parasites. Growing of drug unresponsiveness in leishmaniasis patients necessitates the development of new drugs and accordingly a suitable assay is needed for evaluation of any modalities. The aim of this study was to compare four drug assays methods, agar dilution, broth dilution, cylinder plate and disk diffusion, for evalu...

a collection of enterococci  spp. (about 96 isolates) were isolated from two iranian raw milk cheeses, namely lighvan and koozeh, and subsequently, identified as enterococcus faecium , enterococcus faecalis , enterococcus durans , enterococcus casseliflavus and enterococcus italicus by 16s rdna sequencing. all of the 96 isolates were subjected to agar-spot and well-diffusion assay in order to d...