Seven laboratories participated in a collaborative study to evaluate the Autobac 1 system. Results obtained with this assay system were compared to those obtained by the standardized Bauer-Kirby disk diffusion test, and each of these two methods was compared to the agar dilution technique. Comparison of the Autobac 1 and the disk diffusion results from the seven laboratories showed an overall a...

during the past decade, natural plant products as environmentally safe option have received attention for controlling phytopathogenic diseases. investigation of plants containing natural antimicrobial metabolites for plant protection has been recognized as a desirable method of disease control. the fungus fusarium oxysporum causes diseases such as root rot, damping off and fusarium wilt and it ...

OBJECTIVES The Oxoid M.I.C.Evaluator (M.I.C.E; Thermo Fisher Scientific) comprises an antibiotic gradient on a plastic support. We compared its performance with Etest--a similar product--using BSAC agar dilution as a reference. METHODS Parallel MIC tests were performed by M.I.C.Evaluator, Etest and agar dilution on Iso-Sensitest agar. In total, 9354 organism/strip combinations were tested by ...

In the study except a strain of Dermatophilus congolensis isolated from a dog blood suffering from pyrexia, all bacterial isolates from diseased animals were resistant to discs containing 2 microL sage oil. The only sensitive strain of D. congolensis also showed narrow zone (8 mm) of growth inhibition while the sensitive reference strain of E. coli-382 had 10-12 mm zone of growth inhibition aro...

Tribulus terrestris is used as a urinary anti-infective in folk medicine. To validate this use, the in vitro antibacterial activity of methanolic extracts of different parts (fruits, stems plus leaves and roots) of T. terrestris L. growing in Iran was evaluated against four reference bacteria by broth dilution assay and agar diffusion assay. The MIC value of the methanolic extracts of fruits an...

When antibiotic sensitivity of microorganisms is tested by agar dilution, single antibiotics are incorporated in solid media and 'spot' inocula of test strains are applied to the antibiotic media and to a plate without antibiotic. The usual method of quality control is to include control strains of known sensitivity.1 However, control strains are usually sensitive to a number of antibiotics, an...

Complex physalin metabolites present in the capsules of the fruit of Physalis angulata L. have been isolated and submitted to a series of assays of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, S. aureus ATCC 25923, S. aureus ATCC 6538P, Neisseria gonorrhoeae ATCC 49226, Escherichia coli ATCC 8739; E. coli ATCC 25922, Candida albicans ATCC 1...

A method was developed to determine the susceptibility of Haemophilus influenzae to ampicillin, cefamandole, and chloramphenicol by using the MS-2 system (Abbott Laboratories) for determining minimum inhibitory concentrations (MIC). The MS-2 results for 132 strains of H. influenzae were compared with the results of agar disk diffusion, agar dilution, and beta-lactamase tests. Twenty-four strain...

The agar dilution (Waksman, 1943) and the agar diffusion (Foster and Woodruff, 1943b) methods of assay of streptothricin and streptomycin are the procedures most commonly employed and have led to what Waksman calls "dilution units" (Waksman, 1943) and "diffusion units" (Schatz, Bugie, and Waksman, 1944). However, in addition, Waksman speaks of "Escherichia coli units," or "Bacillus subtilis uni...

The minimal inhibitory concentrations of a series of antimicrobial agents for human oral organisms were determined under anaerobic growth conditions by an agar dilution assay. With the exception of black-pigmented Bacteroides spp., minimal inhibitory concentrations for oral isolates were similar to those for non-oral isolates of organisms of the same or closely related species.