background and objectives: urinary tract infections (utis) are of the most common bacterial diseases worldwide. we investigate the antibiotic susceptibility patterns of escherichia coli (e. coli) strains isolated from pediatric patients with community acquired urinary tract infection (uti) to find a clinical guidance for choosing a right empirical antibiotic in these patients. materials and met...

A reasonably precise, reproducible, and sensitive microbiological procedure for directly assaying sulfacytine and other sulfonamides as antibacterially active drugs has been developed by appropriately modifying the standard disc-agar diffusion technique. Blood and urine levels as low as 3 mug/ml may be determined through the use of a strain of Escherichia coli and a chemically defined agar medi...

OBJECTIVES The agar diffusion assay is one method for quantifying the ability of antibiotics to inhibit bacterial growth. Interpretation of results from this assay relies on model-dependent analysis, which is based on the assumption that antibiotics diffuse freely in the solid nutrient medium. In many cases, this assumption may be incorrect, which leads to significant deviations of the predicte...

OBJECTIVES To investigate the reliability of cefoxitin and oxacillin for the detection of mecC-positive Staphylococcus aureus. METHODS The susceptibility to cefoxitin and oxacillin of 62 mecC-positive S. aureus isolates was investigated using broth microdilution, agar dilution, Etest and disc diffusion on different types of media. The data were interpreted for the utility of cefoxitin and oxa...

background :   this study aimed to evaluate antibacterial effect of allium sativum, garlic extract on erythromycin and methicillin resistant bacteria isolated from an operating room in a teaching hospital in tehran, i.r. iran.   methods :   the antibacterial effect of garlic extract was investigated on 70 bacterial strains. the selected isolates were resistant to erythromycin and or methicillin...

A simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of Clostridium botulinum type A. The method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of C. botulinum type A antitoxin. Concentric precipitin zones formed around colonies of C. botulinum type A. Strains...

Acids, alkalies, salts, glucosides, and certain toxins diffuse more quickly into 0.9 per cent. salt solution than into agar-agar and gelatin suspensions. The inhibitory effect of the colloids grows with increase in concentration, which increase affects both the velocity and extent of the diffusion. In the case of gelatin the degree of diffusion is approximately in inverse proportion to the squa...

Different antimicrobial susceptibility testing methods to detect low-level vancomycin resistance in enterococci were evaluated in a Scandinavian multicenter study (n=28). A phenotypically and genotypically well-characterized diverse collection of Enterococcus faecalis (n=12) and Enterococcus faecium (n=18) strains with and without nonsusceptibility to vancomycin was examined blindly in Danish (...

Background and Aims: The study aimed to determine the effectiveness of three medicinal plant extracts on fungi with three methods and to compare methods. Material and methods: This study examined the antifungal properties of cumin (Cuminum cyminum L), ginger (Zingiber officinale Roscoe) and Nafe Venus (Umbilicus intermedius boiss) extracts against fungi including, Aspergillus spp., Penicillium...

BACKGROUND Leishmaniases are a group of diseases caused by Leishmania parasites. Growing of drug unresponsiveness in leishmaniasis patients necessitates the development of new drugs and accordingly a suitable assay is needed for evaluation of any modalities. The aim of this study was to compare four drug assays methods, agar dilution, broth dilution, cylinder plate and disk diffusion, for evalu...