Objective(s) Definite diagnosis of Hirschsprung’s disease (HD) is based on histopathological study, but there are limitations associated with standard histology and histochemistry in this regard. The aim of this study was to investigate calretinin immunostaining patterns in both ganglionic and aganglionic HD intestinal specimens and to compare them with control specimens. Materials and Method...

OBJECTIVES Enteric neuropathies are severe gastrointestinal disorders with unsatisfactory outcomes. We aimed to investigate the potential of enteric neural stem cell therapy approaches for such disorders by transplanting mouse enteric neural crest cells (ENCCs) into ganglionic and aganglionic mouse gut in vivo and analysing functional integration and long-term safety. DESIGN Neurospheres gene...

Hirschsprung's disease is characterized by absence of ganglion cells in submucosal and myenteric plexus of distal bowel. Most cases become manifest during the neonatal period, but in rare instances, this disease is initially diagnosed in adult age. It usually presents as severe constipation with colonic dilatation proximal to the aganglionic segment. The treatment is surgical, removing the agan...

introduction constipation is one of the most common causes of referral to pediatric gastroenterology clinics. the histopathology and immunohistochemistry (ihc) studies indicated that several neurons are currently detectable in motility of the gut. we aimed to evaluate calretinin in the children with chronic constipation. materials and methods in this cohort study, 40 children with chronic const...

Hirschsprung's disease, a congenital condition resulting in functional intestinal obstruction, was seen in 21 children at the University of Calabar Teaching Hospital (UCTH) between January 1996 and December 1998. Late presentation of patients to hospital was observed and was attributed to ignorance and poverty. Patients presented only when repeated herbal enemas no longer offered relief. The ma...

Hirschsprung disease (HSCR, aganglionic megacolon) is the main genetic cause of functional intestinal obstruction with an incidence of 1/5000 live births. This developmental disorder is a neurocristopathy and is characterised by the absence of the enteric ganglia along a variable length of the intestine. In the last decades, the development of surgical approaches has dramatically decreased mort...

The enteric nervous system of vertebrates is derived from neural crest cells that invade the gut wall and generate a highly organised network of enteric ganglia. Among the genes that play an important role in ENS development is c-Ret, mutations of which result in failure of formation of enteric ganglia (intestinal aganglionosis). To further understand the development of the mammalian ENS in gen...

BACKGROUND & AIMS Enteric nervous system stem cells (ENSSCs) provide potential therapeutic tools to replenish absent ganglia in Hirschsprung's disease. Although full-thickness human postnatal gut tissue can be used to generate ENSSCs, reliance on its harvesting from surgical resection poses significant practical limitations. This study aimed to explore whether gut tissue obtained utilizing mini...

BACKGROUND The use of calretinin immunostain (IHC) in the evaluation of rectal suction biopsies for Hirschsprung disease (HD) has been reported by Kapur et al. and others. The first goal of this article is to report our institutional experience with the use of calretinin in specimens for evaluation of HD. The second goal is to describe the pattern of expression of calretinin in the junction of ...

Objective: To determine the frequency of nerve bundle hypertrophy in aganglionic segments relation to site biopsy, along with assessment value Calretinin immunostaining diagnosis suspected cases Hirschsprung disease. Study Design: Cross Sectional study. Setting: Department Pathology, Pakistan Institute Medical Sciences (PIMS) Islamabad. Period: September 2018 March 2019. Material & Methods:...