background: among diverse protein purification systems, affinity chromatography is the most attractive one in the purification process of coagulation factors. coagulation factor vii is a plasma serine protease that has a significant role in natural human hemostasis and its recombinant form such as aryoseventm, has been applied in clinical treatment of bleeding disorders. immunoaffinity chromato...

the effects of allopurinol, acyclovir and theophylline on the activity of adenosine deaminase (ada) were studied in 50 mm sodium phosphate buffer ph 7.5 at 27°c, using a uv– vis spectrophotometer. adenosine deaminase is inhibited by these ligands, via different types of inhibition. allopurinol, as a transition state analog of xanthine oxidase, and acyclovir competitively inhibit the catalytic a...

â  abstract: in this work, the kinetics of ethane hydrate formation has been studied experimentally and a kinetic model based on chemical affinity has been described for predicting the hydrate growth process in the stirred batch reactor at a constant volume. the experiments were done with both pure water and aqueous solution of sodium dodecyl sulfate (sds). the effect of sds on formation kineti...

  Abstract: In this work, the kinetics of ethane hydrate formation has been studied experimentally and a kinetic model based on chemical affinity has been described for predicting the hydrate growth process in the stirred batch reactor at a constant volume. The experiments were done with both pure water and aqueous solution of sodium dodecyl sulfate (SDS). The effect of SDS on formation kineti...

background: there are two subclasses of human iga (iga1 and iga2) that differ in antigenic properties and in chemical composition. the constant domains of α1 and α2 heavy chains have >95% sequence homology though major structural differences exist in the hinge region. quantitation of iga subclass levels depends on the availability of monoclonal antibodies (mabs) specific for conserved conformat...

A central dogma in immunology is that antibody specificity is solely the result of variable (V)-region interactions with an antigen. However, this view is not tenable in light of numerous reports that constant heavy (C(H)) domains can affect binding affinity and specificity and V-region structure. Kinetic and thermodynamic proof for the occurrence of this phenomenon is now available. C(H)-regio...

T cell tolerance depends on the T cell receptor's affinity for peptide/major histocompatibility complex (MHC) ligand; this critical parameter determines whether a thymocyte will be included (positive selection) or excluded (negative selection) from the T cell repertoire. A quantitative analysis of ligand binding was performed using an experimental system permitting receptor-coreceptor interacti...