Sequencing was used to investigate the origin of the D genome of the allopolyploid species Triticum aestivum and Aegilops cylindrica. A 247-bp region of the wheat D-genome Xwye838 locus, encoding ADP-glucopyrophosphorylase, and a 326-bp region of the wheat D-genome Gss locus, encoding granule-bound starch synthase, were sequenced in a total 564 lines of hexaploid wheat (T. aestivum, genome AABB...

plant breeders use rye (secale cereale l.) as a foreign genetical resource tool in increasing wheat genetical variations. 1r chromosome and especially its short arm, 1rs has been introduced into wheat genome frequently. 1rs studies and effective selection is used in cytogenetical, chemicals techniques and dna markers. present study was conducted to investigate the presence of rye chromosome seg...

Studies of postmeiotic chromosomes have been impeded by the thick exine of immature pollen grains. Chromosome staining of immature and adult pollen grains through conventional acetocarmine procedure is tedious and often leads to unsatisfactory results. An acetocarmine stain, used in conjugation with Carnoy’s solution as fixative for 72 hrs or until the chlorophyll-containing tissues of spike be...

studies of postmeiotic chromosomes have been impeded by the thick exine of immature pollen grains. chromosome staining of immature and adult pollen grains through conven-tional acetocarmine procedure is tedious and often leads to unsatisfactory results. an acetocarmine stain, used in conjugation with carnoy’s solution as fixative for 72 hrs or until the chlorophyll-containing tissues of spike b...

Homoeologous metaphase I (MI) associations in hybrids between durum wheat and its wild allotetraploid relatives Aegilops neglecta, Ae. triuncialis and Ae. ventricosa have been characterized by a genomic in situ hybridization procedure that allows simultaneous discrimination of A, B and wild species genomes. Earlier results in equivalent hybrids with the wild species Ae. cylindrica and Ae. genic...

We have recently produced EMS-induced knock-out mutations at the Gc2 locus that were derived from Ae. sharonensis. Our data suggest that the Gc2 mutant is a knock-out of the gene encoding for the breaking agent. For analyzing the interactions of the Gc2 mutants with other Gc genes from different Aegilops species, we have crossed stocks with functional and mutant Gc2 genes with stocks having Gc ...