The replicative responses of suckling and adult rat hepatocytes in primary culture to growth-stimulating factors were compared. By addition of L-proline alone, the [3H]-thymidine labeling of suckling rat hepatocytes was dramatically enhanced, but that of adult ones was not. Epidermal growth factor (EGF), insulin, triiodothyronine (T3) and glucagon also enhanced the labeling of suckling rat hepa...

BACKGROUND It has been shown that hosphodiesterases (PDEs) play an important role in mediating the smooth muscle tone of rat urinary bladder. However, the gene expression profiles of them were still unknown. METHODS Urinary bladder Strips were obtained from both neonatal and adult Sprague-Dawley rats. RT-PCR/western blot and organ bath were used to measure the expression and function of PDEs....

In agreement with previous results (Awapara, 1956), we noted that the taurine level in the liver of the adult female rat is higher than in the adult male rat: 9.44 and 2.08, respectively, expressed as a concentration (mumoles/g of liver). Furthermore, we observed a significant decrease of the taurine level (expressed similarly) in the liver of the lactating rat: 1.84 twenty-one days after the b...

recently we have reported that newborn rats are deficient in the key enzymes involved in the biotransformation of aflatoxin bl (afbl), a known hepatocarcinogen. based on these data, in vivo experiments were carried out in order to investigate the bioavailability of this carcinogen in newborn rat tissues. administration of a single dose (i. p.) of [3hlafb 1 to groups of adult and neonatal rats r...

A short-term deprivation of rat pups from breastfeeding in the early postnatal period leads to MS at age 4-6 months. However, this neonatal model requires optimization and a detailed study into metabolic hormonal parameters adult animals. The aim work was these male rats deprived breast milk 19-21 days, which achieved by treating lactating females with bromocriptine. Adult showed an increased b...

Erythrocytes (RBC) from untrained male Wistar rats and rat glomerular endothelial cells (EC) were used to investigate the effects of acute exercise (speed: 20 m/min, slope: 0, duration: 1 hour) on RBC membrane protein oxidation and adhesion to cultured EC. Experimental animals were divided into juvenile (age 10 weeks) and adult (age 30 weeks) groups for these studies. Immediately following exer...

1. Acetoacetate or dl-beta-hydroxybutyrate increases the rate of oxygen consumption to a smaller extent than that brought about by glucose or pyruvate in adult rat brain-cortex slices but to the same extent as that in infant rat brain-cortex slices. 2. The rate of (14)CO(2) evolution from [1-(14)C]glucose considerably exceeds that from [6-(14)C]glucose in respiring infant rat brain-cortex slice...