Hairy roots culture is a potential system for secondary metabolite production in medicinal plants. In this research, hairy root induction in Echinacea angustifolia was established using Agrobacterium rhizogenes. The effects of dif-ferent A. rhizogenes strains (AR15834, A4, K599 and MSU440) in two solid growth regula-tor-free culture media, half strength MS salts with B5 vitamins and WPM, were e...

explants of leaves, petals, anthers, styles and ovaries of c. speciosus, c. cancellatus, c. gilanicus and c. almehensis were cultured on ls media with three different combinations of naa and bap in the dark. callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. corm explants from these species were also cultured on ms ...

In this study, we report phytoremediation of textile dyes using hairy roots derived through Agrobacterium rhizogenes (NCIM 5140) infection of in vitro leaf and stem explants of a halophyte Sesuvium portulacastrum (L.) L. Leaf explants showed higher frequency of hairy root induction (70%) than stem explants (30%), and maximum number of roots (leaf 42.3 ± 2.4 and stem 50.3 ± 1.7). Transformed nat...

of embryogenic frequency 88.9 and 72.23 were recorded for stem explants. However, the greatest embryogenic calli fresh weights 14.68 and 9.65 (mg/jar) were derived from leaf explants. Moreover, growth of somatic embryos was investigated during 7weeks. The highest regeneration percentage was achieved for stem explants compared other explants and recorded 88.88 and 66.66 for directly or indirectl...

The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA...

necessary conditions for callus production from leaf and stem explants of norfolk island pine (araucaria excelsa l.) have been studied. ms and bm culture media containing 0.2, 0.5, 1, 2 and 5 mg/l naa, iba, 2,4-d and picloram supplemented with 30 g/l sucrose were used. callus was initiated in both culture media only in the presence of picloram and in the dark, but bm culture medium produced mor...

Conservation and propagation of species using biotechnologic tools-such as plant tissue culture-are relevant when natural propagation is hampered for various reasons. In vitro techniques allow mass multiplication and propagation under pathogen-free conditions but also override dependence on season for availability of plant material. Moreover, in vitro genetic manipulation of a species, invariab...