Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...

Adipose tissue is a complex, highly metabolically active tissue. The expansion of adipose mainly affected by two processes: the number adipocytes and change adipocyte size. Adipocyte differentiation regulated multiple factors. 3T3-L1 preadipocyte line has become most widely studied cell for proliferation, regulation related genes. In our previous study, we found that ANGPTL4 was to through PPAR...

OBJECTIVE Previous studies have demonstrated that mice fed a high-fat diet (HFD) develop insulin resistance with proinflammatory macrophage infiltration into white adipose tissue. Concomitantly, adipocytes undergo programmed cell death with the loss of the adipocyte-specific lipid droplet protein perilipin, and the dead/dying adipocytes are surrounded by macrophages that are organized into crow...

background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...

Adipose tissue expansion in obesity involves a series of cycles of adipocyte hyperplasia, hypertrophy and hypoplasia due to alterations in adipogenesis, adipocyte cellular metabolism and cell death, respectively. Increased frequency of these cycles may lead to deterioration of adipocyte function and viability, accelerated exhaustion of the adipocyte progenitor pool and extensive adipose tissue ...

Abstract The average fat mass in adults increases dramatically with age, and older people often suffer from visceral obesity related adverse metabolic disorders. Unfortunately, how aging leads to accumulation is poorly understood. It known that cell (adipocyte) turnover very low young mice, similar humans. Here, we find mice mimic age-related expansion In vivo lineage tracing shows massive adip...

 Objective: In obesity, chronic low grade inflammation, created by induction of pro-inflammatory markers, causes adipocyte dysfunction in adipose tissue. Adipocytes dysfunction is associated with various diseases including insulin resistance and obesity. In obesity, inflammatory factors such as osteopontin (OPN), angiopoietin-like protein 2 (Angptl2) and transforming growth factor-β (TGF-β) are...

M.E. Symonds (ed.), Adipose Tissue Biology, DOI 10.1007/978-1-4614-0965-6_2, © Springer Science+Business Media, LLC 2012 Abstract Adipocyte differentiation is a highly controlled process that has been extensively studied for the last 25 years. Two different kinds of in vitro experimental models, essential in determining the mechanisms involved in adipocyte proliferation, differentiation and adi...

The adipocyte plays a critical role in energy balance. Adipose tissue growth involves an increase in adipocyte size and the formation of new adipocytes from precursor cells. For the last 20 years, the cellular and molecular mechanisms of adipocyte differentiation have been extensively studied using preadipocyte culture systems. Committed preadipocytes undergo growth arrest and subsequent termin...