A nucleoside kinase has been purified about 1680-fold from L1210 leukemia cells with deoxyadenosine as the phosphate acceptor and with adenosine triphosphate as phosphate donor. The molecular weight of the enzyme, determined by Sephadex G-200 column chromatography, was found to be about 100,000. Purified enzyme, after the final step of purification and also after disc gel electrophoresis (Rf 0....

The mechanisms by which adenosine triphosphate, thrombin, and trypsin cause relaxation of vascular smooth muscle were investigated. Relaxation of the rat thoracic aorta with adenosine triphosphate, thrombin, and/or trypsin was associated with increased levels of cyclic guanosine monophosphate in both time- and concentration-dependent manners. Thrombin and trypsin did not alter cyclic adenosine ...

Adenine nucleotides—adenosine monophosphate, diphosphate, and triphosphate—are of utmost importance to all living organisms, where they play a critical role in the energy metabolism are tied allosteric regulation various regulatory enzymes. Adenylate charge represents precise relationship between concentrations adenosine triphosphate indicates amount metabolic available an organism. The experim...

In order to characterize a step in the reaction sequence of sodium and potassium ion-dependent adenosine triphosphatase (EC 3.6.1.3), the binding of radioactive adenosine triphosphate to kidney cell membranes was estimated by flow dialysis in the presence of a chelator of divalent cations, which prevented hydrolysis of the adenosine triphosphate. Adenosine triphosphate was bound to a single sit...

Shock is a pathological condition characterized by imbalance between oxygen demand and supply. Irrespective of the cause, shock leads to state energy failure due decrease in adenosine triphosphate (ATP) production, creation lactic acidosis, as consequence, multiple organ dysfunction [1].

A dual function for adenosine 5'-triphosphate in the regulation of vascular tone is considered. Adenosine 5'-triphosphate can cause vasodilation, acting via P2-purinoceptors located on vascular endothelial cells to release an endothelium-derived relaxing factor which diffuses to the vascular smooth muscle and induces vasodilation. The main source of intraluminal adenosine 5'-triphosphate is lik...

Factors affecting inhibition of myosin ATPase by ATP, such as concentration of the enzyme, of ATP, and of the divalent metals, calcium and magnesium, have been studied and the results are reported here. Experiments were carried out under conditions in which no known magnesium effects occurred, so that only the effect of calcium on the nucleoside triphosphatase activity of myosin A could be qual...