(2005). Green fluorescent protein-tagged adeno-associated virus particles allow the study of cytosolic and nuclear trafficking. Combinatorial engineering of a gene therapy vector: directed evolution of adeno-associated virus.

Vectors based on adeno-associated virus can stably transfer genes by chromosomal integration in recipient cells. In this study we have infected stationary and dividing primary human fibroblast cultures with adeno-associated virus vectors encoding alkaline phosphatase and neomycin phosphotransferase. We find that the transduction frequency of S phase cells is about 200 times that of non-S phase ...

Gene therapy is an experimental treatment being investigated to correct defective genes that are responsible for disease development. To treat cancer and genetic illnesses, researchers looking into a variety of gene techniques. Finding proper vector transfer DNA tissues one the most difficult aspects therapy. Some vectors have issues with infecting both quiescent dividing cells, provoking immun...