objective(s):antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. the aim of this study was to produce polyclonal antibody against a synthetic peptide derived from n-terminal region of β-actin protein to be used as a protein loading control in western ...

Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...

Eukaryotes harbour a conserved signalling pathway, called General Amino Acid Control (GAAC) in Saccharomyces cerevisiae, for overcoming amino acid starvation. Upon starvation, the protein kinase Gcn2, which phosphorylates eukaryotic translation initiation factor eIF2α, becomes stimulated to trigger GAAC response. Genetic studies suggest that Yih1, is yeast homolog of mammalian IMPACT and binds ...

The mechanism that mediates the interaction between contractile ring and plasma membrane during cytokinesis remains elusive. We previously found ERM (Ezrin/Radixin/Moesin) proteins, which usually mediate cellular pole contraction, become over-accumulated at cell equator support furrow ingression upon loss of other actin-membrane associated anillin supervillin. In this study, we addressed molecu...

Abstract BACKGROUND Altered expression of actin subunits is found in multiple cancers. Alpha-cardiac (ACTC1) expressed sonic hedgehog medulloblastoma (SHH MB) and regulates both cell survival migration. We hypothesized that cardiac has a unique structural functional role SHH MB may be post-natal granule progenitors (GCPs) which share similar gene profiles. METHODS Actin subunit protein (ACTC1, ...

Locally distinct, transient realignments of cortical F-actin were found in live ascidian Ciona eggs during the first ooplasmic segregation by using fluorescence polarization-based molecular orientation imaging. These actin are correlated with directional movement egg cytoplasm revealed selective plane illumination microscopy.

?-actinin-2 (?-actn-2) is an F-actin-crosslinking protein, localized in dendritic spines. In vitro studies suggested that it involved spinogenesis, morphogenesis, actin organization, cell migration and anchoring of the NR1 subunit N-methyl-D-aspartate (NMDA) receptors However, little known regarding its function vivo. We examined levels ?-actn-2 expression within dentate gyrus (DG) during devel...

less than 5% of prenatal thymoctes express hla-dr before week 12 of gestation. however, the number of hla-dr- positive cells increases during the late second and third trimesters of development. to determine the role of alpha-smooth muscle actin in fetal thymic hla-dr signaling in different stages of development we examined and compared the immunohistochemical expression of alpha-smooth muscle ...

Biological phenomena induced by terahertz (THz) irradiation are described in recent reports, but underlying mechanisms, structural and dynamical change of specific molecules still unclear. In this paper, we performed time-lapse morphological analysis human cells found that THz halts cell division at cytokinesis. At the end cytokinesis, contractile ring, which consists filamentous actin (F-actin...