BACKGROUND The aim of this study was to evaluate the ability of dual acridine orange/ethidium bromide (AO/EB) staining to detect tumor cell apoptosis. According to apoptosis-associated changes of cell membranes during the process of apoptosis, a clear distinction is made between normal cells, early and late apoptotic cells, and necrotic cells. MATERIAL AND METHODS We cultured human osteosarco...

ABSTRACT: The interaction of two cationic dyes namely, acridine orange and pinacyanol chloride with an anionic polyelectrolyte, heparin, has been investigated by spectrophotometric method.The polymer induced metachromasy in the dyes resulting in the shift of the absorption maxima of the dyes towards shorter wavelengths. The stability of the complexes formed between acridine orange and heparin w...

An electron microscopic technique has been developed to visualize the binding sites of acridine orange to DNA within fixed human leukemic bone marrow cells. This electron microscopic technique provides both higher resolution and increased chemical specificity for discriminating the nuclear binding sites of acridine orange than does the previous fluorescent light microscopy. Acridine orange bind...

The neutrophil count, immature:total neutrophil ratio, C reactive protein assay, nitroblue tetrazolium test and an acridine orange leucocyte cytospin test were evaluated for the diagnosis of neonatal bacteraemia. The acridine orange leucocyte cytospin test gave the highest specificity and positive predictive accuracy, but was less sensitive than the neutrophil count, C reactive protein assay or...

objective: micronuclei are induced in cells by a variety of substances, like uv radiation, infrared rays, x-radiations, and chemicals. among them tobacco- specific nitrosamines have been reported to be potent mutagenic agents which are thought to be responsible for the induction of chromosomal aberrations resulting in production of micronuclei. the main aim of our study is to compare mn frequen...

purpose: to image leukocyte adherence to the vascular endothelium and extravasation into surrounding tissues in neovascularized corneas using acridine orange, a selective dna stain, in rats methods: corneal neovascularization was induced by placing two sutures in the cornea of one eye of 12 long-evans rats. one week later, acridine orange (4 mg/kg) was injected via the tail vein and the eyes we...

The use of digitonin to permeabilize the plasma membrane of Trypanosoma brucei procyclic and bloodstream trypomastigotes allowed the identification of a non-mitochondrial nigericin-sensitive Ca2+ compartment. The proton ionophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) was able to cause Ca2+ release from this compartment, which was also sensitive to sodium orthovanadate. Prein...

Rapid diagnosis is a prerequisite for institution of effective treatment and reducing the mortality and morbidity of falciparum malaria. This study was taken up to compare the efficacy of various rapid methods viz, acridine orange, Plasmodium falciparum histidine rich protein II antigen detection and Field's stain with traditional microscopy i.e, Leishman stain for diagnosing falciparum malaria...

Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.