Background and Aims: In spite of a long tradition of fruit-tree growing in all provinces of Iran, information on pear (Pyrus communis) tree viruses is scant. In order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses. Materials and Methods: Leaf samples of pear showing virus like symptoms wer...

Thermotherapy and chemotherapy in vitro were applied to eliminate Apple chlorotic leaf spot virus (ACLSV) and Prunus necrotic ring spot virus (PNRSV) from myrobalan (Prunus cerasifera var. divaricata Borgh.), PNRSV from ‘Empress’ plum (Prunus domestica L.) and Prune dwarf virus (PDV) from ‘Early Rivers’ sweet cherry (Cerasus avium (L.) Moench.) plants. ELISA assays conducted one year after ther...

Selected problems related to non-uniformity of plant virus sequence variants present in infected plants and imperfectness of current diagnostic methods are illustrated and discussed. A failure of some primers to react (efficiently) with atypical isolates/strains of Apple chlorotic leaf spot virus (ACLSV) and Plum pox virus (PPV) is demonstrated. On the other hand, problematic positive reactions...

Deep sequencing has generated 52 contigs derived from five viruses; Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple green crinkle associated virus (AGCaV), and Apricot latent virus (ApLV) were identified from eight apple samples showing small leaves and/or growth retardation. Nucleotide (nt) sequence identity of the assembled con...

The development of a real-time 5' nuclease RT-PCR assay for the detection of apple chlorotic leaf spot virus (ACLSV) from infected plant material is described. A short fluorogenic 3' minor groove binder-DNA hydrolysis probe was used to circumvent genome variability between isolates and target a short conserved sequence. The covalent attachment of the minor groove binder moiety at the 3' end of ...

Several viruses can cause devastating diseases in domestic apple (Malus x domestica Brokh.). Major viruses affecting apple production in New York are apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV). These viruses are common and generally latent; in other words, they do not cause acute symptoms on most commercial cultivars. However, when...

Apples are the third most produced fruit in world, behind bananas and oranges, terms of volume. Over past ten years, Serbia's apple production has nearly doubled. less subjected to viral viroid attacks than other species. Most viruses cause no visible symptoms on plant's organs, trees either seem be good condition or, case sensitive cultivars, their yield is reduced. Apple stem pitting virus (A...

Amaç: Bu çalışmada, Adıyaman ili genelinde yetişen badem ağaçlarında apple mosaic virus (ApMV), prunus necrotic ring spot (PNRSV), prune dwarf (PDV), plum pox (PPV) ve chlorotic leaf (ACLSV)’lerinin Double Antibody Sandwich-Enzyme Linked Immuno Sorbent Assay (DAS-ELISA) Reverse Transcription-Polymerase Chain Reaction (RT-PCR) yöntemleriyle belirlenmesi, elde edilen virüs izolatlarının filogenet...